Holkem Augusto Tasch, Favaro-Trindade Carmen Sílvia, Lacroix Monique
Universidade de São Paulo, Faculdade de Zootecnia e Engenharia de Alimentos, Av. Duque de Caxias Norte, 225, CEP 13635-900, Pirassununga, São Paulo, Brazil; Research Laboratory in Sciences Applied to Food, INRS Armand-Frappier Health and Biotechnology Centre, Canadian Irradiation Centre, Institute of Nutrition and Functional Foods (INAF), 531 Boulevard des Prairies, Laval, Quebec H7V 1B7, Canada.
Universidade de São Paulo, Faculdade de Zootecnia e Engenharia de Alimentos, Av. Duque de Caxias Norte, 225, CEP 13635-900, Pirassununga, São Paulo, Brazil.
Food Res Int. 2020 Aug;134:109274. doi: 10.1016/j.foodres.2020.109274. Epub 2020 Apr 26.
Bifidobacterium animalis subsp. lactis (BLC1) and proanthocyanidin-rich cinnamon extract (PRCE) have many beneficial health properties. However, they are very sensitive materials; co-encapsulation is one alternative to protect them. Therefore, the objective of this work was to evaluate the anticancer properties of free PRCE and in combination with BLC1 and the resistance of these free and co-encapsulated materials under in vitro simulated gastrointestinal conditions. In terms of anticancer proprieties, PRCE had an IC value close to 30 mg extract/mL for Hepa 1c1c7 and HT-29 cells and resulted in a significantly higher percentage (p ≤ 0.05) of total apoptotic and necrotic cells compared to treatment in combination with BLC1 (PRCE + BLC1), with values above 31.66% in both cells. For the quinone reductase (QR) assay, there was a significant increase only for PRCE + BLC1 treatment, with a fold induction of 5.11 ± 0.56 for HT-29. The resistance of the encapsulated materials was greater than for the free form after 240 min of simulated gastrointestinal conditions. The combination of these materials in a microcapsule is advantageous because it protects them under gastrointestinal conditions, allowing them to be released into the intestine and act in the early stages of colon cancer.
动物双歧杆菌乳亚种（BLC1）和富含原花青素的肉桂提取物（PRCE）具有许多有益健康的特性。然而，它们是非常敏感的物质；共包封是保护它们的一种方法。因此，本研究的目的是评估游离PRCE以及与BLC1联合使用时的抗癌特性，以及这些游离和共包封物质在体外模拟胃肠道条件下的耐受性。在抗癌特性方面，PRCE对Hepa 1c1c7和HT - 29细胞的IC值接近30 mg提取物/mL，与BLC1联合处理（PRCE + BLC1）相比，导致总凋亡和坏死细胞的百分比显著更高（p≤0.05），两种细胞中的值均高于31.66%。对于醌还原酶（QR）测定，仅PRCE + BLC1处理有显著增加，HT - 29的诱导倍数为5.11±0.56。在模拟胃肠道条件240分钟后，包封材料的耐受性大于游离形式。这些物质在微胶囊中的组合是有利的，因为它在胃肠道条件下保护它们，使其能够释放到肠道中并在结肠癌的早期阶段发挥作用。
