Laboratorio de Referencia Nacional de Leishmaniasis, Instituto Nacional de Salud, Lima, Perú.
Laboratorio de Biotecnología y Biología Molecular, Instituto Nacional de Salud, Lima, Perú.
Rev Peru Med Exp Salud Publica. 2020 Jan-Mar;37(1):87-92. doi: 10.17843/rpmesp.2020.371.4514. Epub 2020 Jun 8.
In Peru, leishmaniasis is a metaxenic disease that represents a serious public health problem, due to its wide distribution and the number of people in danger of contracting the disease, being the vulnerable population mainly those with low economic resources. The study was conducted from patients who were derived to Peru's National Institute of Health between 2006 and 2011 so that the specialized diagnosis could be carried out. The identification of the species of infectious Leishmania was developed through the analysis of the High-Resolution Melting Analysis obtained from the genomic DNA of promastigotes and amastigotes, which allows to identify the species of Leishmania (Viannia) braziliensis, Leishmania (V.) guyanensis, Leishmania (V.) peruviana as more prevalent, in addition to Leishmania (V.) lainsoni and Leishmania (L.) amazonensis.
在秘鲁,利什曼病是一种外生性疾病,由于其分布广泛以及面临患病风险的人数众多,成为一个严重的公共卫生问题,弱势群体主要是那些经济资源匮乏的人群。本研究的对象是 2006 年至 2011 年期间被转诊到秘鲁国家卫生研究所的患者,以便进行专业诊断。通过对从原虫和无鞭毛体的基因组 DNA 中获得的高分辨率熔解分析进行分析,确定了感染性利什曼原虫的种类,这使得能够鉴定出更为流行的利什曼(Viannia)巴西利什曼原虫、利什曼(V.)圭亚那利什曼原虫、利什曼(V.)秘鲁利什曼原虫,此外还有利什曼(V.)lainsoni 和利什曼(L.)amazonensis。