巴西里约热内卢州利什曼原虫（Viannia）巴西利ensis 临床分离株中利什曼 RNA 病毒 1 的首次报告。
First report of Leishmania RNA virus 1 in Leishmania (Viannia) braziliensis clinical isolates from Rio de Janeiro State - Brazil.
机构信息
Fundação Oswaldo Cruz-Fiocruz, Instituto Oswaldo Cruz, Laboratório de Biologia Molecular e Doenças Endêmicas, Rio de Janeiro, RJ, Brasil.
Fundação Oswaldo Cruz-Fiocruz, Instituto Oswaldo Cruz, Laboratório Interdisciplinar de Pesquisas Médicas, Rio de Janeiro, RJ, Brasil.
出版信息
Mem Inst Oswaldo Cruz. 2022 Aug 22;117:e210107. doi: 10.1590/0074-02760210107. eCollection 2022.
BACKGROUND
Leishmania parasites carry a double-stranded RNA virus (Leishmania RNA virus - LRV) that has been divided in LRV1 and LRV2.
OBJECTIVES
Leishmania (Viannia) braziliensis clinical isolates were assessed in order to determine LRV presence.
METHODS
Two-round polymerase chain reaction (PCR and nested PCR) was performed to detect LRV1 or LRV2 in L. (V.) braziliensis clinical isolates (n = 12).
FINDINGS
LRV1 was detected in three clinical isolates which was phylogenetically related to other sequences reported from other American tegumentary leishmaniasis (ATL) endemic areas of Brazil. Patients infected with L. (V.) braziliensis LRV-negative showed only cutaneous lesions while LRV-positive reported different manifestations.
MAIN CONCLUSION
Data presented here show for the first time that LRV1 is circulating in L. (V.) braziliensis clinical isolates from Rio de Janeiro State in Brazil.
背景
利什曼原虫携带一种双链 RNA 病毒（利什曼 RNA 病毒 - LRV），已分为 LRV1 和 LRV2。
目的
评估利什曼（Viannia）巴西利ensis 临床分离株以确定 LRV 的存在。
方法
采用两轮聚合酶链反应（PCR 和巢式 PCR）检测 L.（V.）巴西利ensis 临床分离株中的 LRV1 或 LRV2（n = 12）。
发现
在三个临床分离株中检测到了 LRV1，这些分离株与来自巴西其他皮肤利什曼病（ATL）流行地区的其他报告序列具有系统发育关系。感染 L.（V.）巴西利ensis LRV 阴性的患者仅出现皮肤损伤，而 LRV 阳性的患者则出现不同的表现。
主要结论
这里提供的数据首次表明，LRV1 在巴西里约热内卢州的 L.（V.）巴西利ensis 临床分离株中循环。
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