LncRNA CTBP1-AS2 通过调控 miR-623/Cyclin D1 轴促进肝癌细胞增殖。
LncRNA CTBP1-AS2 Promotes Cell Proliferation in Hepatocellular Carcinoma by Regulating the miR-623/Cyclin D1 Axis.
机构信息
Department of Pleurisy, Changchun Infectious Diseases Hospital, Changchun, China.
Department of General Surgery, Xuhui District Central Hospital of Shanghai, Shanghai, China.
出版信息
Cancer Biother Radiopharm. 2020 Dec;35(10):765-770. doi: 10.1089/cbr.2019.3375. Epub 2020 Jun 9.
This study investigated the role of lncRNA CTBP1-AS2 in hepatocellular carcinoma (HCC). The authors found that CTBP1-AS2 was upregulated in HCC by analyzing TCGA dataset. The downregulation of CTBP1-AS2 in HCC was confirmed by measuring the expression level of CTBP1-AS2 in both HCC and nontumor tissues from HCC patients. MiR-623 is predicted to target CTBP1-AS2, while it failed to downregulate its expression. Interestingly, CTBP1-AS2 overexpression led to the upregulation of cyclin D1, a target of miR-623. CCK-8 analysis showed that CTBP1-AS2 and cyclin D1 overexpression promoted the proliferation of HCC cells. MiR-623 overexpression played an opposite role and reduced the effects of CTBP1-AS2 and cyclin D1 overexpression. Therefore, CTBP1-AS2 promotes cell proliferation in HCC by regulating the miR-623/cyclin D1 axis.
本研究探讨了长链非编码 RNA CTBP1-AS2 在肝细胞癌（HCC）中的作用。作者通过分析 TCGA 数据集发现 CTBP1-AS2 在 HCC 中上调。通过测量 HCC 患者的 HCC 和非肿瘤组织中 CTBP1-AS2 的表达水平，证实了 CTBP1-AS2 在 HCC 中的下调。MiR-623 被预测为靶向 CTBP1-AS2，但未能下调其表达。有趣的是，CTBP1-AS2 的过表达导致 miR-623 的靶标 cyclin D1 的上调。CCK-8 分析表明，CTBP1-AS2 和 cyclin D1 的过表达促进了 HCC 细胞的增殖。MiR-623 的过表达则起到相反的作用，降低了 CTBP1-AS2 和 cyclin D1 过表达的效果。因此，CTBP1-AS2 通过调节 miR-623/cyclin D1 轴促进 HCC 中的细胞增殖。
Zotero 插件