Department of Pleurisy, Changchun Infectious Diseases Hospital, Changchun, China.
Department of General Surgery, Xuhui District Central Hospital of Shanghai, Shanghai, China.
Cancer Biother Radiopharm. 2020 Dec;35(10):765-770. doi: 10.1089/cbr.2019.3375. Epub 2020 Jun 9.
This study investigated the role of lncRNA CTBP1-AS2 in hepatocellular carcinoma (HCC). The authors found that CTBP1-AS2 was upregulated in HCC by analyzing TCGA dataset. The downregulation of CTBP1-AS2 in HCC was confirmed by measuring the expression level of CTBP1-AS2 in both HCC and nontumor tissues from HCC patients. MiR-623 is predicted to target CTBP1-AS2, while it failed to downregulate its expression. Interestingly, CTBP1-AS2 overexpression led to the upregulation of cyclin D1, a target of miR-623. CCK-8 analysis showed that CTBP1-AS2 and cyclin D1 overexpression promoted the proliferation of HCC cells. MiR-623 overexpression played an opposite role and reduced the effects of CTBP1-AS2 and cyclin D1 overexpression. Therefore, CTBP1-AS2 promotes cell proliferation in HCC by regulating the miR-623/cyclin D1 axis.
本研究探讨了长链非编码 RNA CTBP1-AS2 在肝细胞癌（HCC）中的作用。作者通过分析 TCGA 数据集发现 CTBP1-AS2 在 HCC 中上调。通过测量 HCC 患者的 HCC 和非肿瘤组织中 CTBP1-AS2 的表达水平，证实了 CTBP1-AS2 在 HCC 中的下调。MiR-623 被预测为靶向 CTBP1-AS2，但未能下调其表达。有趣的是，CTBP1-AS2 的过表达导致 miR-623 的靶标 cyclin D1 的上调。CCK-8 分析表明，CTBP1-AS2 和 cyclin D1 的过表达促进了 HCC 细胞的增殖。MiR-623 的过表达则起到相反的作用，降低了 CTBP1-AS2 和 cyclin D1 过表达的效果。因此，CTBP1-AS2 通过调节 miR-623/cyclin D1 轴促进 HCC 中的细胞增殖。
