lncRNA DLGAP1-AS2 通过调控 miR-154-5p 的甲基化抑制肝癌细胞迁移和侵袭。
lncRNA DLGAP1-AS2 Knockdown Inhibits Hepatocellular Carcinoma Cell Migration and Invasion by Regulating miR-154-5p Methylation.
机构信息
The First Department of General Surgery, Affiliated Hospital of Chengde Medical University, Chengde City, Hebei Province, 067000, China.
Department of Clinical Laboratory, Affiliated Hospital of Chengde Medical University, Chengde City, Hebei Province, 067000, China.
出版信息
Biomed Res Int. 2020 Oct 9;2020:6575724. doi: 10.1155/2020/6575724. eCollection 2020.
OBJECTIVE
DLGAP1-AS2 has been characterized as an oncogenic lncRNA in glioma. Our preliminary microarray analysis revealed the altered expression of DLGAP1-AS2 in hepatocellular carcinoma (HCC), but the role of DLGAP1-AS2 in HCC remains unknown.
METHOD
Expression of DLGAP1-AS2 and miR-154-5p in paired HCC and nontumor tissues from 62 HCC patients was determined by RT-qPCR. The 62 HCC patients were followed up for 5 years to analyze the prognostic value of DLGAP1-AS2 for HCC. DLGAP1-AS2 knockdown and miR-154-5p overexpression was achieved in HCC cells to study the relationship between them. Methylation of miR-154-5p was analyzed by methylation-specific PCR. Cell proliferation was analyzed by CCK-8 assay.
RESULTS
DLGAP1-AS2 was upregulated in HCC and predicted poor survival. miR-154-5p was downregulated in HCC and inversely correlated with DLGAP1-AS2. In HCC cells, DLGAP1-AS2 knockdown resulted in the upregulation of miR-154-5p expression and decreased methylation of miR-154-5p gene. Transwell assay showed that DLGAP1-AS2 knockdown and miR-154-5p overexpression inhibited cell invasion and migration, and the combination of LGAP1-AS2 knockdown and miR-154-5p overexpression showed stronger effects.
CONCLUSION
DLGAP1-AS2 knockdown may inhibit HCC cell migration and invasion by regulating miR-154-5p methylation.
目的
DLGAP1-AS2 已被鉴定为神经胶质瘤中的致癌 lncRNA。我们的初步微阵列分析显示，在肝细胞癌（HCC）中 DLGAP1-AS2 的表达发生改变，但 DLGAP1-AS2 在 HCC 中的作用尚不清楚。
方法
通过 RT-qPCR 测定 62 例 HCC 患者配对 HCC 组织和非肿瘤组织中 DLGAP1-AS2 和 miR-154-5p 的表达。对 62 例 HCC 患者进行 5 年随访，分析 DLGAP1-AS2 对 HCC 的预后价值。在 HCC 细胞中敲低 DLGAP1-AS2 和过表达 miR-154-5p 以研究它们之间的关系。通过甲基化特异性 PCR 分析 miR-154-5p 的甲基化。通过 CCK-8 测定分析细胞增殖。
结果
DLGAP1-AS2 在 HCC 中上调，预测预后不良。miR-154-5p 在 HCC 中下调，与 DLGAP1-AS2 呈负相关。在 HCC 细胞中，敲低 DLGAP1-AS2 导致 miR-154-5p 表达上调，miR-154-5p 基因甲基化降低。Transwell 实验表明，DLGAP1-AS2 敲低和 miR-154-5p 过表达抑制细胞侵袭和迁移，DLGAP1-AS2 敲低和 miR-154-5p 过表达的联合作用更强。
结论
DLGAP1-AS2 敲低可能通过调节 miR-154-5p 甲基化抑制 HCC 细胞迁移和侵袭。
Zotero 插件