Kiefer M, Neff N, Chamberlin M J
J Virol. 1977 May;22(2):548-52. doi: 10.1128/JVI.22.2.548-552.1977.
Bacterial mutations of known rho genotype (psu-1 through psu-4) were shown to have no effect on transcriptional termination at the termination site at the end of the early region of T3 and T7 DNAs. Transcriptional termination was assayed in these bacterial mutants by comparing the patterns of late protein production produced by T3 and T7 amber I bacteriophage infection. All the rho (psu) mutants tested showed the same pattern of late protein production as the wild-type strain (rho+ or psu+) after T3 or T7 amber I infection. The presence of the mutant rho allele during bacteriophage infection, therefore, did not allow the host RNA polymerase to read through the terminator located at approximately 20% of the T3 and T7 genomes. These results suggest that rho factor may not be involved in reading of the T3 and T7 20% terminators in vivo.
已知rho基因型（psu - 1至psu - 4）的细菌突变体对T3和T7 DNA早期区域末端终止位点处的转录终止没有影响。通过比较T3和T7琥珀型I噬菌体感染产生的晚期蛋白质产生模式，在这些细菌突变体中测定转录终止。在T3或T7琥珀型I感染后，所有测试的rho（psu）突变体显示出与野生型菌株（rho +或psu +）相同的晚期蛋白质产生模式。因此，在噬菌体感染期间突变rho等位基因的存在不允许宿主RNA聚合酶通读位于T3和T7基因组约20％处的终止子。这些结果表明rho因子可能不参与体内T3和T7 20％终止子的通读。
