有证据表明利福平可刺激大肠杆菌中转录终止子的通读，包括rpoBC操纵子的弱化子。
Evidence that rifampicin can stimulate readthrough of transcriptional terminators in Escherichia coli, including the attenuator of the rpoBC operon.
作者信息
Newman A J, Ma J C, Howe K M, Garner I, Hayward R S
出版信息
Nucleic Acids Res. 1982 Nov 25;10(22):7409-24. doi: 10.1093/nar/10.22.7409.
Abstract
The genes encoding the beta and beta' subunits of RNA polymerase in E.coli, rpoB and rpoC, lie downstream of at least two ribosomal protein genes, rplJ (encoding L10) and rplL (L7/12), in a common operon. All four genes are served by promoter PL10, and an attenuator (partial terminator) of transcription, t1, lies between rplJL and rpoBC. Treatment of E.coli with rifampicin, under conditions producing partial inhibition of general RNA synthesis, can stimulate transcription of rpoBC. We have investigated the locus of this effect by fusing PL10 and t1 separately to galK, in suitable plasmids. Our studies of these fusions, and similar fusions involving transcriptional terminators derived from coliphage T7, indicate that low concentrations of rifampicin cause increased readthrough of several different transcriptional terminators in E.coli in vivo, including rpo t1. We discuss whether or not this unspecific mechanism is solely responsible for the observed stimulatory effects of the drug on rpoBC transcription.
摘要
大肠杆菌中编码RNA聚合酶β和β'亚基的基因rpoB和rpoC，位于一个共同操纵子中至少两个核糖体蛋白基因rplJ（编码L10）和rplL（L7/12）的下游。所有这四个基因都由启动子PL10调控，并且转录衰减子（部分终止子）t1位于rplJL和rpoBC之间。在产生对一般RNA合成部分抑制的条件下，用利福平处理大肠杆菌可刺激rpoBC的转录。我们通过在合适的质粒中将PL10和t1分别与galK融合，研究了这种效应的位点。我们对这些融合体以及涉及来自噬菌体T7的转录终止子的类似融合体的研究表明，低浓度的利福平会导致大肠杆菌体内几种不同转录终止子的通读增加，包括rpo t1。我们讨论了这种非特异性机制是否是该药物对rpoBC转录所观察到的刺激作用的唯一原因。
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Evidence that rifampicin can stimulate readthrough of transcriptional terminators in Escherichia coli, including the attenuator of the rpoBC operon.有证据表明利福平可刺激大肠杆菌中转录终止子的通读，包括rpoBC操纵子的弱化子。
Nucleic Acids Res. 1982 Nov 25;10(22):7409-24. doi: 10.1093/nar/10.22.7409.
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