Cao Tingshuai, Cao Yuanchao, Wang Hongqiang, Wang Peitao, Wang Xinsheng, Niu Haitao, Shao Cuihua
Department of Urology, Jinan Central Hospital Affiliated to Shandong University, Jinan, People's Republic of China.
Department of Urology, The Affiliated Hospital of Qingdao University, Qingdao, People's Republic of China.
Dose Response. 2020 May 18;18(2):1559325820926745. doi: 10.1177/1559325820926745. eCollection 2020 Apr-Jun.
Although bisphenol A (BPA) has been associated with impaired spermatogenesis, the mechanisms remain unclear. Tight junction occludin plays important roles in spermatogenesis. The objective of the present study was to explore the effects of BPA exposure in adolescent mice. Male mice were orally treated with low-dose (0.05 mg/kg/d), middle-dose (5.0 mg/kg/d), or high-dose (50 mg/kg/d) BPA in corn oil from postnatal day (PND) 35 to 65. Animals were killed on PND 65 and PND 125. On PND 65, the sperm count, sperm motility, and the expression of occludin showed a dose-related decline. On PND 125, the sperm count, sperm motility, and the expression of occludin were in recovery. However, there remained significant decreases in these parameters in the 50 mg/kg/d group on PND 125 compared with the control. The dose-related effects on the measured parameters and occludin expression suggest an early suppressive or damaging effect on the blood-testis barrier followed by recovery after dosing ceased. At a BPA dose of 50 mg/kg/d, recovery did not occur, suggesting that higher doses of BPA may cause irreversible damage to reproduction in male mice.
尽管双酚A（BPA）与精子发生受损有关，但其机制仍不清楚。紧密连接蛋白occludin在精子发生中起重要作用。本研究的目的是探讨双酚A暴露对青春期小鼠的影响。从出生后第35天（PND）至65天，雄性小鼠经口给予低剂量（0.05 mg/kg/d）、中剂量（5.0 mg/kg/d）或高剂量（50 mg/kg/d）的双酚A玉米油溶液。在PND 65和PND 125处死动物。在PND 65时，精子计数、精子活力和occludin的表达呈剂量依赖性下降。在PND 125时，精子计数、精子活力和occludin的表达有所恢复。然而，与对照组相比，在PND 125时，50 mg/kg/d组的这些参数仍有显著下降。对测量参数和occludin表达的剂量相关影响表明，双酚A对血睾屏障有早期抑制或损伤作用，给药停止后可恢复。在双酚A剂量为50 mg/kg/d时，未出现恢复，这表明较高剂量的双酚A可能对雄性小鼠的生殖造成不可逆的损害。
