College of Animal Science and Technology, Northwest A&F University, Yangling, Shaanxi 712100, China.
College of Animal Science and Technology, Northwest A&F University, Yangling, Shaanxi 712100, China.
Ecotoxicol Environ Saf. 2019 Apr 30;171:475-483. doi: 10.1016/j.ecoenv.2019.01.007. Epub 2019 Jan 9.
Bisphenol A (BPA), an environmental contaminant, has been shown to disturb the dynamics of Sertoli cell blood-testis barrier (BTB) in mammal testis. However, the effects of BPA on Sertoli cell barrier (SC barrier) were little known in fish to date. To evaluate the potential mechanism of reproductive toxicity of BPA, we studied the damage of SC barrier using in vivo models. In this study, male adult rare minnow Gobiocypris rarus were exposed to 15 μg/L BPA for 7-35 days. Gonadal histology and the integrity of SC barrier were analyzed. Meanwhile, the expressions of SC barrier -associated proteins, tumor necrosis factor (TNFα) content, and the mRNA expressions of genes in the mitogen activated protein kinase (MAPK) pathway were detected. Histological analysis demonstrated 15 μg/L BPA promoted the infiltration of inflammatory cells in fish testes after 7-days exposure. The biotin tracer assay showed that 7-days BPA exposure increased permeability for spermatid cysts. In addition, the BPA treatment caused increased TNFα in testis, which was reportedly related to SC barrier impairment. The expressions of Occludin and β-Catenin protein were significantly decreased in the testes after 7- and 21-days exposure. BPA also altered the mRNA expressions of occludin, β-catenin, p38 MAPK and JNK. Therefore, the detrimental effects of BPA on reproduction of male fish may attribute to the disturbed expressions of SC junction proteins.
双酚 A（BPA）作为一种环境污染物，已被证实会扰乱哺乳动物睾丸中支持细胞血睾屏障（BTB）的动态平衡。然而，迄今为止，BPA 对鱼类支持细胞屏障（SC 屏障）的影响知之甚少。为了评估 BPA 的生殖毒性的潜在机制，我们使用体内模型研究了 SC 屏障的损伤。在这项研究中，雄性成年稀有鮈鲫（Gobiocypris rarus）暴露于 15μg/L BPA 中 7-35 天。分析了性腺组织学和 SC 屏障的完整性。同时，检测了与 SC 屏障相关的蛋白、肿瘤坏死因子（TNFα）含量以及丝裂原激活蛋白激酶（MAPK）通路中基因的 mRNA 表达。组织学分析表明，15μg/L BPA 暴露 7 天后会促进鱼类睾丸中炎症细胞的浸润。生物素示踪剂测定表明，7 天 BPA 暴露会增加精子细胞囊的通透性。此外，BPA 处理导致睾丸中 TNFα 的增加，据报道这与 SC 屏障损伤有关。Occludin 和β-Catenin 蛋白的表达在 7 天和 21 天暴露后睾丸中明显下降。BPA 还改变了 occludin、β-catenin、p38 MAPK 和 JNK 的 mRNA 表达。因此，BPA 对雄性鱼类生殖的有害影响可能归因于 SC 连接蛋白表达的紊乱。
