Graduate Institute of Food Science and Technology, National Taiwan University, 1, Sec 4, Roosevelt Road, Taipei 10617, Taiwan.
Department of Food Science, College of Agriculture, National Pingtung University of Science and Technology, 1, Shuefu Road, Neipu, Pingtung 91201, Taiwan.
Food Chem. 2020 Nov 15;330:127244. doi: 10.1016/j.foodchem.2020.127244. Epub 2020 Jun 3.
The aim of this study is to simultaneously evaluate anti-oxidative and anti-inflammatory activities of the hop extracts by different solvents. Hop water extract (HWE) and hop ethanol extracts (HEEs) were prepared by extracting hop pellets with hot water at 90 °C and ethanol solutions (55%, 75%, and 95%), respectively. Bioactive compound such as α-acid, β-acid, total phenolic, and total flavonoid contents were determined. All the HEEs showed higher anti-oxidative activities than the HWEs. The HEEs showing the highest anti-oxidative activities are different in the experiments with different free radicals. For anti-inflammatory activities, both the HWE and HEEs decreased NO productions. HWE decreased TNF-α and IL-6 secretion but showed no effect on IL-1β, while HEEs decreased IL-1β and IL-6 secretion but increased TNF-α secretion. Except for TNF-α secretion, the HEEs showed higher anti-inflammatory activities than the HWE. Future work is to explore the possible mechanism to improve the ethanol extraction procedure.
本研究旨在通过不同溶剂同时评估啤酒花提取物的抗氧化和抗炎活性。采用 90°C 的热水和 55%、75%和 95%的乙醇溶液分别提取啤酒花颗粒，制备啤酒花水提取物（HWE）和啤酒花乙醇提取物（HEE）。测定了生物活性化合物如α-酸、β-酸、总酚和总黄酮的含量。所有 HEEs 的抗氧化活性均高于 HWEs。在不同自由基的实验中，表现出最高抗氧化活性的 HEEs 不同。对于抗炎活性，HWE 和 HEEs 均降低了 NO 的产生。HWE 降低了 TNF-α和 IL-6 的分泌，但对 IL-1β没有影响，而 HEEs 降低了 IL-1β和 IL-6 的分泌，但增加了 TNF-α的分泌。除 TNF-α分泌外，HEE 的抗炎活性均高于 HWE。未来的工作是探索改善乙醇提取程序的可能机制。
