Inserm, CHU Nantes, Université de Nantes, Centre de Recherche en Transplantation et Immunologie, UMR 1064, ITUN, Nantes, France.
Labex IGO, Nantes, France.
Eur J Immunol. 2020 Nov;50(11):1757-1769. doi: 10.1002/eji.201948489. Epub 2020 Jun 23.
Regulatory B cells (Bregs) have the ability to regulate inflammation in various pathological situations, making them key players in immune regulation. Several mechanisms have been described and we recently identified a GZMB expressing Breg population in kidney transplanted patients who tolerate a kidney graft. To further investigate their biology and mechanisms, we conducted a transcriptomic analysis by RNAseq of these cells and we performed the first weighted meta-analysis of publicly available transcriptomic data from published Breg studies both in humans and mice. We identified two distinct and unique transcriptional signatures of 126 and 93 genes, respectively, associated with these Bregs. While we highlighted genes coding for proteins with potent involvement in regulatory functions, proliferation, and coding for transcription factors, the comparison between humans and mice did not allow identifying a common pattern. Thus, our results suggest distinct species-restricted Breg transcriptional signatures in humans and mice.
调节性 B 细胞（Bregs）具有在各种病理情况下调节炎症的能力，使其成为免疫调节的关键参与者。已经描述了几种机制，我们最近在接受肾移植的患者中鉴定出一种表达 GZMB 的 Breg 群体，这些患者能够耐受肾移植。为了进一步研究它们的生物学和机制，我们通过 RNAseq 对这些细胞进行了转录组分析，并对来自人类和小鼠的已发表 Breg 研究的公开转录组数据进行了首次加权荟萃分析。我们分别鉴定出与这些 Bregs 相关的两个独特的转录特征，分别由 126 和 93 个基因组成。虽然我们强调了编码具有强大调节功能、增殖和编码转录因子的蛋白质的基因，但人类和小鼠之间的比较并未确定共同的模式。因此，我们的结果表明人类和小鼠存在独特的受物种限制的 Breg 转录特征。
