抗菌肽 cathelicidin-BF 对断奶后腹泻仔猪腹泻控制、免疫应答、肠道炎症和肠道屏障功能的影响。
Effects of antimicrobial peptide cathelicidin-BF on diarrhea controlling, immune responses, intestinal inflammation and intestinal barrier function in piglets with postweaning diarrhea.
机构信息
College of Veterinary Medicine, South China Agricultural University, Wushan Road, Tianhe district, Guangzhou 510642, China.
State Key Laboratory of Livestock and Poultry Breeding, Ministry of Agriculture and Rural Affairs Key Laboratory of Animal Nutrition and Feed Science in South China, Guangdong Key Laboratory of Animal Breeding and Nutrition, Institute of Animal Science, Guangdong Academy of Agricultural Sciences, 1 Dafeng 1st Street, Guangzhou 510640, China.
出版信息
Int Immunopharmacol. 2020 Aug;85:106658. doi: 10.1016/j.intimp.2020.106658. Epub 2020 Jun 9.
The objective was to evaluate the effects of antimicrobial peptide cathelicidin-BF (C-BF) treatment on diarrhea controlling, immune responses, intestinal inflammation, and intestinal barrier function in piglets with postweaning diarrhea. In this study, fifty-four weaned piglets with diarrhea were selected and treated with saline (control), C-BF or norfloxacin nicotinic (NFN) for 7 days. Here, we investigated the effects of C-BF on diarrhea controlling, growth performance, serum immune indicators, intestinal morphology, intestinal inflammation, and intestinal epithelial barrier function in the weaned piglets with diarrhea. The results showed that C-BF treatment decreased (P < 0.05) diarrheal index and increased (P < 0.05) average daily gain (ADG) and average daily feed intake (ADFI) compared with control group. C-BF treatment decreased (P < 0.05) levels of serum blood urea nitrogen (BUN) and aspartate aminotransferase (AST), but increased (P < 0.05) levels of serum A/G and alkaline phosphatase (ALP) compared with control group. The concentrations of IL-6, IL-8, tumor necrosis factor-α (TNF-α), and IgG were lower (P < 0.05), but IgA was greater (P < 0.05) for piglets treated by C-BF compared with those in control group. C-BF and NFN treatment decreased (P < 0.05) IL-6, IL-8, IL-22, IL-10 and transforming growth factor-β (TGF-β) production in the jejunum and ileum compared with the control group. C-BF treatment increased the expression levels of zonula occluden-1 (ZO-1), Occludin, and Claudin-1 in the jejunum and colon compared with the control group and the NFN group. In conclusion, these data indicate that C-BF treatment may be an effective therapeutic strategy for controlling post-weaning diarrhea, improving immune responses, attenuating intestinal inflammation and enhancing intestinal barrier function in piglets with postweaning diarrhea.
本研究旨在评估抗菌肽 cathelicidin-BF (C-BF) 对断奶后腹泻仔猪腹泻控制、免疫反应、肠道炎症和肠道屏障功能的影响。选择 54 头腹泻断奶仔猪，分别用生理盐水（对照组）、C-BF 或诺氟沙星烟酸（NFN）处理 7 天。在这里，我们研究了 C-BF 对腹泻控制、生长性能、血清免疫指标、肠道形态、肠道炎症和肠道上皮屏障功能的影响。结果表明，与对照组相比，C-BF 处理降低（P < 0.05）腹泻指数，提高（P < 0.05）平均日增重（ADG）和平均日采食量（ADFI）。C-BF 处理降低（P < 0.05）血清血尿素氮（BUN）和天冬氨酸转氨酶（AST）水平，但提高（P < 0.05）血清 A/G 和碱性磷酸酶（ALP）水平。与对照组相比，C-BF 处理仔猪的 IL-6、IL-8、肿瘤坏死因子-α（TNF-α）和 IgG 浓度较低（P < 0.05），但 IgA 浓度较高（P < 0.05）。C-BF 和 NFN 处理降低（P < 0.05）空肠和回肠中 IL-6、IL-8、IL-22、IL-10 和转化生长因子-β（TGF-β）的产生。与对照组和 NFN 组相比，C-BF 处理增加了空肠和结肠中 zonula occluden-1 (ZO-1)、Occludin 和 Claudin-1 的表达水平。综上所述，这些数据表明 C-BF 处理可能是一种有效的治疗策略，可控制断奶后腹泻，改善免疫反应，减轻肠道炎症，增强断奶后腹泻仔猪的肠道屏障功能。
Zotero 插件