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Suppression of polarity of insertion mutations within the gal operon of E. coli.

作者信息

Besemer J, Herpers M

出版信息

Mol Gen Genet. 1977 Mar 16;151(3):295-304. doi: 10.1007/BF00268793.

DOI:10.1007/BF00268793
PMID:325374
Abstract

Phenotypic revertants of galOP::IS1 and galOP::IS2 mutations have been isolated after mutagenesis with nitrosoguanidine, they are probably caused by mutations in gene suA. The polarity suppressor mutations described in this study and a known mutation in gene suA isolated by D. Morse (Morse and Guertin, 1972) suppress polarity caused by IS1 more effectively than that caused by IS2 or IS4. Furthermore, suppressibility is influenced by the site and orientation of IS integration. The synthesis of the three enzymes in galOP::IS suA double mutants is constitutive and the ratio of the three enzymes is altered in comparison to the wild type. The reasons for constitutive synthesis of the galactose enzymes and for the altered ratio of enzyme synthesis are discussed.

摘要

相似文献

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Suppression of polarity of insertion mutations within the gal operon of E. coli.
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2
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引用本文的文献

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Characterization of two mutations in the Escherichia coli galE gene inactivating the second galactose operator and comparative studies of repressor binding.大肠杆菌galE基因中使第二个半乳糖操纵基因失活的两个突变的表征及阻遏物结合的比较研究。
EMBO J. 1983;2(12):2129-35. doi: 10.1002/j.1460-2075.1983.tb01713.x.
2
A control system which causes alternating turn-off/turn-on of transcription on insertion element IS1.一种控制系统，其导致插入元件IS1上的转录交替关闭/开启。
Mol Gen Genet. 1983;192(1-2):61-5. doi: 10.1007/BF00327647.
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An IS4-encoded protein is synthesized in minicells.

本文引用的文献

1
HEREDITARY DEFECTS IN GALACTOSE METABOLISM IN ESCHERICHIA COLI MUTANTS, I. DETERMINATION OF ENZYME ACTIVITIES.大肠杆菌突变体中半乳糖代谢的遗传性缺陷，I. 酶活性的测定
Proc Natl Acad Sci U S A. 1959 Dec;45(12):1776-86. doi: 10.1073/pnas.45.12.1776.
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Effect of different conformations of galactose messenger RNA on gene expression and messenger half-life in vitro.半乳糖信使核糖核酸不同构象对体外基因表达和信使半衰期的影响。
Mol Gen Genet. 1975;136(1):41-54. doi: 10.1007/BF00275447.
3
Control of inducibility of enzymes of the galactose sequence in Escherichia coli.
一种由IS4编码的蛋白质在微小细胞中合成。
Mol Gen Genet. 1981;182(2):183-8. doi: 10.1007/BF00269656.
4
The sequence of IS4.IS4的序列。
Mol Gen Genet. 1981;181(2):169-75. doi: 10.1007/BF00268423.
5
Deletions and DNA rearrangements within the transposable DNA element IS2. A model for the creation of palindromic DNA by DNA repair synthesis.转座DNA元件IS2内的缺失和DNA重排。通过DNA修复合成产生回文DNA的模型。
Nucleic Acids Res. 1980 Dec 11;8(23):5825-33. doi: 10.1093/nar/8.23.5825.
6
The structure of unstable constitutive revertants of mutant galOP-308::IS2-I.突变体galOP-308::IS2-I的不稳定组成型回复突变体的结构
Mol Gen Genet. 1980;179(2):391-7. doi: 10.1007/BF00425470.
7
IS4 at its chromosomal site in E. coli K12.大肠杆菌K12染色体位点上的IS4。
Mol Gen Genet. 1980;178(2):285-91. doi: 10.1007/BF00270474.
8
Antitermination of transcription from an Escherichia coli ribosomal RNA promoter.来自大肠杆菌核糖体RNA启动子的转录抗终止作用。
Proc Natl Acad Sci U S A. 1984 Nov;81(21):6789-93. doi: 10.1073/pnas.81.21.6789.
9
Efficient read-through of Tn9 and IS1 by RNA polymerase molecules that initiate at rRNA promoters.由在rRNA启动子处起始的RNA聚合酶分子对Tn9和IS1进行高效通读。
J Bacteriol. 1983 Feb;153(2):672-84. doi: 10.1128/jb.153.2.672-684.1983.
10
A transcriptional terminator sequence in the prokaryotic transposable element IS1.原核转座因子IS1中的一个转录终止子序列。
Mol Gen Genet. 1987 Mar;206(3):485-90. doi: 10.1007/BF00428889.
大肠杆菌中半乳糖序列酶诱导性的控制
Proc Natl Acad Sci U S A. 1962 Jan 15;48(1):32-40. doi: 10.1073/pnas.48.1.32.
4
[REGULATORY MECHANISMS IN THE BIOSYNTHESIS OF THE ENZYMES OF GALACTOSE METABOLISM IN ESCHERICHIA COLI K 12. I. THE INDUCED BIOSYNTHESIS OF GALACTOKINASE AND THE SIMULTANEOUS INDUCTION OF THE ENZYMATIC SEQUENCE].[大肠杆菌K12中半乳糖代谢酶生物合成的调控机制。I. 半乳糖激酶的诱导生物合成及酶序列的同步诱导]
J Mol Biol. 1963 Aug;7:164-82. doi: 10.1016/s0022-2836(63)80044-3.
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Production of galactokinase by an O degree-mutant of the galactose operon in Escherichia coli.大肠杆菌中半乳糖操纵子O突变体对半乳糖激酶的产生
Biochem Biophys Res Commun. 1965 Sep 8;20(5):647-51. doi: 10.1016/0006-291x(65)90449-3.
6
The galactose operon of E. coli K-12. II. A deletion analysis of operon structure and polarity.大肠杆菌K-12的半乳糖操纵子。II. 操纵子结构与极性的缺失分析。
Genetics. 1969 Jun;62(2):249-64. doi: 10.1093/genetics/62.2.249.
7
The galactose operon of E. coli K-12. I. Structural and pleiotropic mutations of the operon.大肠杆菌K-12的半乳糖操纵子。I. 操纵子的结构和多效性突变
Genetics. 1969 Jun;62(2):231-47. doi: 10.1093/genetics/62.2.231.
8
Termination factor for RNA synthesis.RNA合成的终止因子。
Nature. 1969 Dec 20;224(5225):1168-74. doi: 10.1038/2241168a0.
9
Negative control of the galactose operon in E. coli.
Mol Gen Genet. 1968;102(1):79-88. doi: 10.1007/BF00341872.
10
DNA directed peptide synthesis. V. The cell-free synthesis of a polypeptide with beta-galactosidase activity.
Biochem Biophys Res Commun. 1968 Aug 21;32(4):710-4. doi: 10.1016/0006-291x(68)90297-0.