Rauf Abdur, Bawazeer Saud, Naseer Muhammad, Alhumaydhi Fahad A, Aljohani Abdullah S M, Habib Aamir, Khan Raheem, Jehan Urooj, Qureshi Muhammad Nasimullah, Khan Majid, Farooq Umar, Kassenov Amirzhan, Shariati Mohammad Ali
Department of Chemistry, University of Swabi, Swabi, Pakistan.
Department of Pharmaceutical Chemistry, Faculty of Pharmacy, Umm Al-Qura University, Makkah, Saudi Arabia.
Nat Prod Res. 2021 Dec;35(23):5434-5439. doi: 10.1080/14786419.2020.1779264. Epub 2020 Jun 15.
The current study aims at exploring enzyme inhibition of four species of medicinal herbs, namely and . Plant selection was done on the basis of their therapeutic uses by local practitioners. The crude methanolic extracts of these plants were tested for their -glycosidase and urease enzyme inhibition potential. The observed urease inhibitory potential for the crude extract of , and were 8.3 ± 0.33 μg, 6.98 ± 0.98 μg and 9.56 ± 1.43 μg, respectively while the did not show any inhibition. In addition, the IC value for Thiourea was 22.3 ± 1.14 μg. The crude extracts of were shown to inhibit -glycosidase activity with an IC value of 630.3 ± 0.03 μg, 700.7 ± 2.43 μg, 430.4 ± 3.97 μg, and the standard (acarbose) 880 ± 1.03 M, respectively. Based on the TLC profile, the extract of was subjected to column chromatography and the major component named rhein () was identified. Compound exhibited excellent urease and -glycosidase inhibitory activity with an IC value of 7.4 ± 0.32 and 622.3 ± 1.03 M, respectively.
