Natural and Medical Sciences Research Center, University of Nizwa, P.O. Box 33, Postal Code 616, Birkat Al Mauz, Nizwa, Oman.
Department of Biological Sciences & Chemistry, College of Arts and Sciences, University of Nizwa, Oman.
Biomed Res Int. 2022 Jul 15;2022:7880387. doi: 10.1155/2022/7880387. eCollection 2022.
The recent study was designed to explore , , , and using methanolic (MeOH) extract. Their subfractions were examined against urease, carbonic anhydrase II (CA-II), -glucosidase enzymes, and free radicals scavenging significance based on local practices via standard methods. Significance potential against the urease enzyme was presented by ethyl acetate fraction (EtOAc) of with ( = 125 ± 1.75 g/mL), whereas the ( = 146 ± 1.39 g/mL) in the EtOAc was found efficient to scavenge the free radicals. Besides, that appreciable capacity was observed by the , , , and as compared to the standard acarbose (IC = 377.24 ± 1.14 g/mL). Maximum significance was noticed in methanolic (MeOH) extract of and presented carbonic anhydrase CA-II (IC = 5.1 ± 0.20 g/mL) inhibition as compared to the standard (acetazolamide). We are reporting, for the first time, the CA-II inhibition of all the selected medicinal plants and -glucosidase, urease, and antioxidant activities of the . Thus, further screening is needed to isolate the promising bioactive ingredients which act as an alternative remedy to scavenge the free radicals, antiulcer, and act as a potential source to develop new antidiabetic drugs for controlling postprandial blood sugar as well as carbonic anhydrase inhibitors.
本研究旨在利用甲醇（MeOH）提取物探索、、、和的抑制作用。根据当地实践，通过标准方法，对其各馏分进行了对脲酶、碳酸酐酶 II（CA-II）、-葡萄糖苷酶的抑制作用和自由基清除意义的研究。乙酸乙酯（EtOAc）馏分（ = 125 ± 1.75 g/mL）对脲酶的抑制作用呈现出显著的抑制效果，而 EtOAc 中的（ = 146 ± 1.39 g/mL）对自由基的清除作用具有较高的效率。此外，与标准阿卡波糖（IC = 377.24 ± 1.14 g/mL）相比，、、、和也表现出了相当的能力。与标准品相比，、、、和的甲醇（MeOH）提取物对碳酸酐酶 CA-II（IC = 5.1 ± 0.20 g/mL）的抑制作用最大。我们首次报道了所有选定药用植物的 CA-II 抑制作用，以及的 -葡萄糖苷酶、脲酶和抗氧化活性。因此，需要进一步筛选以分离出有希望的生物活性成分，这些成分可以作为清除自由基、抗溃疡的替代疗法，并作为开发新的抗糖尿病药物的潜在来源，以控制餐后血糖和碳酸酐酶抑制剂。
