Laboratory of Nano-regenerative Medical Engineering, Department of Biomedical Science, College of Life Science, CHA University, 618, CHA Biocomplex, Sampyeong-Dong, Bundang-gu, Seongnam-si 13488, Republic of Korea.
ACS Appl Mater Interfaces. 2020 Jul 8;12(27):30750-30760. doi: 10.1021/acsami.0c05286. Epub 2020 Jun 24.
Electrically conductive materials can stimulate stem cells through electric shock and thereby contribute to the regulation of cell proliferation and differentiation. Recently, polymer-metal complexes composed of polyaniline and gold nanoparticles have emerged as novel candidates for use in regenerative medicine. By mixing two different materials, such composites maximize the benefits while alleviating the disadvantages of using either material alone. Based on their excellent conductivity, these complexes can be applied to nerve regeneration using stem cells. In this study, we investigated a method for producing hybrid nanocomposites by complexing gold nanoparticles to polyaniline and tested the resultant composites in a model of nerve regeneration. We manipulated the shape, size, and electrical conductivity of the hybrid composites by compounding the component materials at various ratios. The most efficient nanocomposite was named conductive reinforced nanocomposites (CRNc's). When the CRNc was delivered directly to cells, no cytotoxicity was observed. After the intracellular delivery of the CRNc, the stem cells were electrically stimulated using an electroporator. As a result of performing mRNA-sequencing (Seq) analysis after electrical stimulation (ES) of the CRNc-internalized cells, it was confirmed that the CRNc-internalized cells have a pattern similar to that of the positive group-induced neuron cells. In particular, microtubule-associated protein 2 is more than twice that of the control group (negative control), and the nerve fiber protein is strongly expressed as in the positive control group. In addition, we verified that neural differentiation progressed by monitoring the growth of neurites from stem cells. Together, these findings show that the CRNc can be used to induce the formation of neuron-like cells by applying ES to stem cells.
导电材料可以通过电击刺激干细胞，从而有助于调节细胞增殖和分化。最近，由聚苯胺和金纳米粒子组成的聚合物-金属配合物作为再生医学的新型候选材料出现。通过混合两种不同的材料，这些复合材料可以最大限度地发挥优势，同时缓解单独使用任何一种材料的缺点。基于其优异的导电性，这些配合物可用于使用干细胞进行神经再生。在这项研究中，我们通过将金纳米粒子与聚苯胺复合来研究生产杂化纳米复合材料的方法，并在神经再生模型中测试了所得复合材料。我们通过以不同比例复合组成材料来操纵杂化复合材料的形状、大小和导电性。效率最高的纳米复合材料被命名为导电增强纳米复合材料（CRNc）。当将 CRNc 直接递送到细胞中时，没有观察到细胞毒性。在将 CRNc 递送到细胞内后，使用电穿孔器对干细胞进行电刺激。对 CRNc 内化细胞进行电刺激（ES）后的 mRNA 测序（Seq）分析结果证实，CRNc 内化细胞具有类似于阳性诱导神经元细胞的模式。特别是，微管相关蛋白 2 是对照组（阴性对照）的两倍多，神经纤维蛋白的表达与阳性对照组相似。此外，我们通过监测干细胞的突起生长来验证神经分化的进展。总之，这些发现表明，通过对干细胞施加 ES，CRNc 可用于诱导神经元样细胞的形成。
