Department of Medical Sciences, Graduate School of Medicine, Korea University, Seoul, South Korea.
Department of Neurosurgery, Guro Hospital, College of Medicine, Korea University, Seoul, Korea.
Sci Rep. 2020 Jun 15;10(1):9655. doi: 10.1038/s41598-020-66689-0.
Intervertebral disc (IVD) degeneration with chronic low back pain is associated with neo-vascularisation into the deeper IVD regions. During this process, endothelial cells (ECs), which are primarily responsible for angiogenesis, interact with the adjacent annulus fibrosus (AF) cells, which are the first line of defence against the invasion of vascular structures into deeper IVD regions. However, the accumulation of inflammatory and catabolic enzymes that results from this interaction promotes matrix degradation and an inflammatory response. Thus, regulating the production of these mediators and catabolic enzymes could ameliorate IVD degeneration. Photobiomodulation (PBM) therapy is a non-invasive stimulation known to have biologically beneficial effects on wound healing, tissue repair, and inflammation. Here, we examined the effects of PBM, administered at various wavelengths (645, 525, and 465 nm) and doses (16, 32, and 64 J/cm), on EC-stimulated human AF cells. Our results show that PBM selectively inhibited the EC-mediated production of inflammatory mediators, catabolic enzymes, and neurotrophins by human AF cells in a dose- and wavelength-dependent manner. These results suggest that PBM could be a superior and advanced treatment strategy for IVD degeneration.
椎间盘（IVD）退化伴慢性下腰痛与深部 IVD 区域的新生血管形成有关。在此过程中，内皮细胞（ECs）主要负责血管生成，与相邻的纤维环（AF）细胞相互作用，而后者是防止血管结构侵入深部 IVD 区域的第一道防线。然而，这种相互作用导致的炎症和分解代谢酶的积累促进了基质降解和炎症反应。因此，调节这些介质和分解代谢酶的产生可以改善 IVD 退化。光生物调节（PBM）疗法是一种非侵入性刺激，已知对伤口愈合、组织修复和炎症具有有益的生物学作用。在这里，我们研究了 PBM 在不同波长（645、525 和 465nm）和剂量（16、32 和 64J/cm）下对 EC 刺激的人 AF 细胞的影响。我们的结果表明，PBM 以剂量和波长依赖的方式选择性抑制了 EC 介导的人 AF 细胞产生炎症介质、分解代谢酶和神经营养因子。这些结果表明，PBM 可能是 IVD 退化的一种优越和先进的治疗策略。
