Yadav Suraj Singh, Singh Manish Kumar, Dwivedi Pradeep, Mandal Raju Kumar, Usman Kauser, Khattri Sanjay, Pant Kamlesh Kumar
Department of Pharmacology and Therapeutics, King George's Medical University, Lucknow, Uttar Pradesh, India.
Department of Medical Education and Research, King Khalid University Hospital, Riyadh, Saudi Arabia.
Toxicol Int. 2014 Jan;21(1):107-11. doi: 10.4103/0971-6580.128818.
A consortium of metabolic risk factors accelerate the onset of diabetes, heart disease, stroke, and certain cancers. Proteolytic enzymes like matrix metalloproteinases (MMP) are regulated by a group of endogenous proteins called tissue inhibitors of metalloproteinases (TIMP). These TIMPs binds to active and alternate sites of activated MMPs and facilitate regulation. Impaired expression of MMPs may have a significant contribution in the pathogenesis of many tissues-destructive processes like tumor progression and cardiovascular and metabolic disorders.
This case control study lays stress on the possible role of impaired levels of circulating MMP-2 and 9 in metabolic syndrome (MetS). The age, sex-matched 388 subjects with 190 newly diagnosed patients, and 198 healthy controls were recruited. To screen the patients with MetS, biochemical analysis of patients for impaired glucose level, hypertension, body mass index (BMI), and lipid profile was performed. The circulating level of MMP-2 and -9 in serum was analyzed by enzyme-linked immunosorbent assay (ELISA) in all patients and control.
All metabolic risk factors were statistically significant (P < 0.01) in patients against control group. The serum MMP-2 and -9 level was significantly higher (P < 0.001) in patients having MetS as compared with control group.
Similar trend was observed in gender wise analysis of serum MMP level. Higher MMP level alteration observed in male patients as compared with female patients.
一组代谢风险因素会加速糖尿病、心脏病、中风和某些癌症的发病。像基质金属蛋白酶（MMP）这样的蛋白水解酶受一组称为金属蛋白酶组织抑制剂（TIMP）的内源性蛋白质调节。这些TIMP与活化MMP的活性位点和交替位点结合并促进调节。MMP表达受损可能在许多组织破坏性过程如肿瘤进展以及心血管和代谢紊乱的发病机制中起重要作用。
本病例对照研究着重探讨循环中MMP-2和9水平受损在代谢综合征（MetS）中的可能作用。招募了年龄、性别匹配的388名受试者，其中190名是新诊断患者，198名是健康对照。为筛查MetS患者，对患者进行了血糖水平受损、高血压、体重指数（BMI）和血脂谱的生化分析。所有患者和对照均通过酶联免疫吸附测定（ELISA）分析血清中MMP-2和-9的循环水平。
与对照组相比，患者的所有代谢风险因素均具有统计学意义（P < 0.01）。与对照组相比，患有MetS的患者血清MMP-2和-9水平显著更高（P < 0.001）。
在血清MMP水平的性别分析中观察到类似趋势。与女性患者相比，男性患者中观察到更高水平的MMP改变。
