Huang Hai-Tao, Xu Yi-Ming, Ding Sheng-Guang, Yu Xiao-Qiang, Wang Fei, Wang Hai-Feng, Tian Xue, Zhong Chong-Jun
Department of Thoracic and Cardiovascular Surgery, Nantong First People's Hospital, Nantong, Jiangsu Province, China.
Division of Geriatrics, Tongji Hospital, School of Medicine, Tongji University, Shanghai, China.
Arch Med Sci. 2020 Mar 9;16(4):931-940. doi: 10.5114/aoms.2020.93535. eCollection 2020.
Lung cancer is the leading cause of cancer-associated mortality worldwide. Recently, long non-coding RNAs (lncRNAs) have been studied as key regulators in some biological processes. Of note, the molecular mechanism and prognostic value of lncRNAs in non-small cell lung cancer (NSCLC) have largely remained unclear.
In this study, we compared the PTTG3P expression levels between lung cancer and normal lung samples by analyzing 5 public datasets (GSE18842, GSE19804, GSE27262, GSE30219, and GSE19188). Next, pentose phosphate pathway and co-expression networks were constructed to identify key targets of lncRNA PTTG3P. Furthermore, gene ontology (GO) and Kyoto Encyclopedia of Genes and Genomes (KEGG) pathway analysis were performed to explore the potential roles of lncRNA PTTG3P. Moreover, we constructed PTTG3P-mediated ceRNA networks in lung adenocarcinoma (LUAD) and lung squamous cell carcinoma (LUSC).
In the present study, our analysis showed that PTTG3P expression was higher in high T stage LUAD and LUSC samples, as well as high N stage NSCLC tissues. Of note, we found that higher PTTG3P expression is correlated with shorter survival time in NSCLC patients by analyzing Kaplan-Meier plotter datasets. We found that PTTG3P was significantly associated with NSCLC cell proliferation regulation by affecting a series of cell cycle related biological processes.
Bioinformatics analysis showed that PTTG3P was associated with NSCLC cell proliferation. These results suggested that PTTG3P could serve as a new therapeutic and prognostic target for NSCLC.
肺癌是全球癌症相关死亡的主要原因。最近，长链非编码RNA（lncRNAs）已被研究作为某些生物学过程中的关键调节因子。值得注意的是，lncRNAs在非小细胞肺癌（NSCLC）中的分子机制和预后价值在很大程度上仍不清楚。
在本研究中，我们通过分析5个公共数据集（GSE18842、GSE19804、GSE27262、GSE30219和GSE19188）比较了肺癌和正常肺样本之间的PTTG3P表达水平。接下来，构建磷酸戊糖途径和共表达网络以鉴定lncRNA PTTG3P的关键靶点。此外，进行基因本体（GO）和京都基因与基因组百科全书（KEGG）通路分析以探索lncRNA PTTG3P的潜在作用。此外，我们在肺腺癌（LUAD）和肺鳞状细胞癌（LUSC）中构建了PTTG3P介导的ceRNA网络。
在本研究中，我们的分析表明，PTTG3P表达在高T期LUAD和LUSC样本以及高N期NSCLC组织中较高。值得注意的是，通过分析Kaplan-Meier绘图仪数据集，我们发现较高的PTTG3P表达与NSCLC患者较短的生存时间相关。我们发现PTTG3P通过影响一系列与细胞周期相关的生物学过程与NSCLC细胞增殖调节显著相关。
生物信息学分析表明PTTG3P与NSCLC细胞增殖相关。这些结果表明PTTG3P可作为NSCLC的新治疗和预后靶点。
