Portillo-Esquivel Luis Eduardo, Nanduri Vibudha, Zhang Feng, Liang Wenbin, Zhang Boyang
Department of Chemical Engineering, McMaster University, 1280 Main Street West, Hamilton, ON, L8S 4L8, Canada.
School of Biomedical Engineering, McMaster University, 1280 Main Street West, Hamilton, ON, L8S 4L8, Canada.
Adv Healthc Mater. 2020 Jul;9(14):e2000358. doi: 10.1002/adhm.202000358. Epub 2020 Jun 16.
Tissue engineering holds promise to replace damaged tissues for repair of vital organs in the human body. In cardiac repairs specifically, approaches are developed for intramyocardial delivery of cells and the epicardial delivery of tissue-engineered cardiac patches, providing benefit of cell localization and tissue structure, respectively. However, to improve cell retention and integration, there is a need for the intramyocardial delivery of functional tissues while preserving anisotropic muscle alignment. Here, a biodegradable z-wire scaffold that supports the scalable gel-free production of an array of functional cardiac tissues in a 384-well plate format is developed. The z-wire scaffold design supports cellular alignment, provides tunable mechanical support, and allows for tissue contraction. When the scaffold is imparted with magnetic properties, individual tissues can be assembled with macroscopic alignment under magnetic guidance. When used in combination with a customized surgical delivery tool, z-wire tissues can be injected directly into the myocardial wall, with controlled tissue orientation according to the injection path. This modular tissue engineering approach, in combination with the use of smart scaffolds, can expand opportunity in functional tissue delivery.
组织工程有望替代受损组织，用于修复人体重要器官。特别是在心脏修复方面，已开发出心肌内细胞递送和组织工程心脏补片的心外膜递送方法，分别提供了细胞定位和组织结构的益处。然而，为了提高细胞保留和整合能力，需要在保持各向异性肌肉排列的同时进行功能性组织的心肌内递送。在此，开发了一种可生物降解的z线支架，该支架支持以384孔板形式可扩展地无凝胶生产一系列功能性心脏组织。z线支架设计支持细胞排列，提供可调机械支撑，并允许组织收缩。当支架具有磁性时，单个组织可在磁引导下以宏观排列方式组装。当与定制的手术递送工具结合使用时，z线组织可直接注射到心肌壁中，并根据注射路径控制组织方向。这种模块化组织工程方法与智能支架的使用相结合，可以扩大功能性组织递送的机会。
