Shoba Ekambaram, Lakra Rachita, Kiran Manikantan Syamala, Korrapati Purna Sai
Biological Materials Laboratory, CSIR - Central Leather Research Institute, Chennai, 600 020, India.
Biological Materials Laboratory, CSIR - Central Leather Research Institute, Chennai, 600 020, India.
J Mech Behav Biomed Mater. 2020 Sep;109:103822. doi: 10.1016/j.jmbbm.2020.103822. Epub 2020 May 7.
Chronic periodontal disease affect the tissues supporting around the teeth like gingival tissue, connective tissue, alveolar bone and periodontal ligaments. Hitherto, periodontal treatment was targeted to selectively repopulate the defect site with cell that has capability to regenerate lost tissue by promoting the concept of guided tissue regeneration but it requires second surgery due to non- biodegradability. The use of polymeric biodegradable nanofibrous coated scaffold that have the ability to deliver bioactives required for regeneration to occur is relatively a newer concept. The functionalization of polymeric scaffold with Bromelain and magnesium doped hydroxyapatite nanoparticle enhanced the mechanical, physico-chemical, thermal and biological properties of the scaffold by imitating the intricate extracellular matrix (ECM) architecture which provided the necessary bioactive cues that offered control over cellular functions by showing antibacterial potential, hemocompatibility and increasing the proliferation and migration rate in vitro. In addition, in ovo chicken chorioallantoic membrane assay and ex vivo aortic ring assay confirmed the efficacy of the developed scaffold by encouraging angiogenesis required for maintaining its viability after implanting onto the infected area. Further, the scaffold positively interacted with the host and actively contributed to the process of tissue regeneration in vivo in Wistar rat model.
慢性牙周病会影响牙齿周围的支持组织，如牙龈组织、结缔组织、牙槽骨和牙周韧带。迄今为止，牙周治疗的目标是通过引导组织再生的概念，选择性地用具有再生丢失组织能力的细胞重新填充缺损部位，但由于不可生物降解性，这需要二次手术。使用具有递送再生所需生物活性物质能力的可生物降解聚合物纳米纤维涂层支架是一个相对较新的概念。用菠萝蛋白酶和镁掺杂羟基磷灰石纳米颗粒对聚合物支架进行功能化，通过模仿复杂的细胞外基质（ECM）结构，增强了支架的机械、物理化学、热学和生物学性能，该结构提供了必要的生物活性线索，通过显示抗菌潜力、血液相容性并提高体外增殖和迁移率，对细胞功能进行控制。此外，鸡胚绒毛尿囊膜试验和离体主动脉环试验通过促进植入感染区域后维持其活力所需的血管生成，证实了所开发支架的有效性。此外，在Wistar大鼠模型中，该支架与宿主发生积极相互作用，并在体内积极促进组织再生过程。
