葡聚糖基质纳米复合材料中镁掺杂羟基磷灰石的新物理化学分析

New Physico-Chemical Analysis of Magnesium-Doped Hydroxyapatite in Dextran Matrix Nanocomposites.

作者信息

Predoi Daniela, Ciobanu Steluta Carmen, Iconaru Simona Liliana, Ţălu Ştefan, Ghegoiu Liliana, Matos Robert Saraiva, da Fonseca Filho Henrique Duarte, Trusca Roxana

机构信息

National Institute of Materials Physics, Atomistilor Street, No. 405A, P.O. Box MG 07, 077125 Magurele, Romania.

The Directorate of Research, Development and Innovation Management (DMCDI), Technical University of Cluj-Napoca, 15 Constantin Daicoviciu St., 400020 Cluj-Napoca, Romania.

出版信息

Polymers (Basel). 2023 Dec 29;16(1):125. doi: 10.3390/polym16010125.

DOI:10.3390/polym16010125

PMID:38201790

原文链接:https://pmc.ncbi.nlm.nih.gov/articles/PMC10780894/

Abstract

The new magnesium-doped hydroxyapatite in dextran matrix (10MgHApD) nanocomposites were synthesized using coprecipitation technique. A spherical morphology was observed by scanning electron microscopy (SEM). The X-ray diffraction (XRD) characterization results show hydroxyapatite hexagonal phase formation. The element map scanning during the EDS analysis revealed homogenous distribution of constituent elements of calcium, phosphor, oxygen and magnesium. The presence of dextran in the sample was revealed by Fourier transform infrared (FTIR) spectroscopy. The antimicrobial activity of the 10MgHAPD nanocomposites was assessed by in vitro assays using ATCC 25923, ATCC 27853, ATCC 25175, ATCC 33277 and ATCC 10231 microbial strains. The results of the antimicrobial assays highlighted that the 10MgHApD nanocomposites presented excellent antimicrobial activity against all the tested microorganisms and for all the tested time intervals. Furthermore, the biocompatibility assays determined that the 10MgHApD nanocomposites did not exhibit any toxicity towards Human gingival fibroblast (HGF-1) cells.

摘要

采用共沉淀技术合成了新型葡聚糖基质中掺杂镁的羟基磷灰石（10MgHApD）纳米复合材料。通过扫描电子显微镜（SEM）观察到其呈球形形态。X射线衍射（XRD）表征结果表明形成了羟基磷灰石六方相。能谱分析（EDS）期间的元素图谱扫描显示钙、磷、氧和镁等组成元素分布均匀。通过傅里叶变换红外（FTIR）光谱证实了样品中葡聚糖的存在。使用ATCC 25923、ATCC 27853、ATCC 25175、ATCC 33277和ATCC 10231微生物菌株通过体外试验评估了10MgHAPD纳米复合材料的抗菌活性。抗菌试验结果突出表明，10MgHApD纳米复合材料在所有测试时间间隔内对所有测试微生物均表现出优异的抗菌活性。此外，生物相容性试验确定10MgHApD纳米复合材料对人牙龈成纤维细胞（HGF-1）没有任何毒性。