Department of Biology, The University of Western Ontario , London, Ontario, Canada.
London Research and Development Centre, Agriculture and Agri-Food Canada , London, Ontario, Canada.
Plant Signal Behav. 2020 Aug 2;15(8):1774715. doi: 10.1080/15592324.2020.1774715. Epub 2020 Jun 16.
Histone deacetylases (HDACs) play an important role in plant stress response. In , which is model species for molecular biology research on monocot plants, the histone deacetylase BdHD1, homologous to of the RPD3/HDA1 class, functions as a positive regulator in the plant drought stress response. has been found to interact with transcription factors to regulate gene expression. However, the drought-responsive transcription factors that interact with BdHD1 have not been identified yet. Previously, we identified BdWRKY24 and BdMYB22 as drought responsive transcription factors in . In this study, we used yeast two-hybrid (Y2 H) and bimolecular fluorescence complementation (BiFC) assays to show that BdHD1 interacts with BdWRKY24 and BdMYB22. Our findings provides a base to investigate BdHD1-transcription factor complexes in the context of drought stress response in .
组蛋白去乙酰化酶 (HDACs) 在植物应激反应中发挥着重要作用。在 ，它是单子叶植物分子生物学研究的模式物种，组蛋白去乙酰化酶 BdHD1 与 RPD3/HDA1 类的 同源，作为植物干旱胁迫反应的正调节剂。已发现它与转录因子相互作用以调节基因表达。然而，与 BdHD1 相互作用的干旱响应转录因子尚未被鉴定。之前，我们在 中鉴定了 BdWRKY24 和 BdMYB22 作为干旱响应转录因子。在这项研究中，我们使用酵母双杂交 (Y2H) 和双分子荧光互补 (BiFC) 测定来表明 BdHD1 与 BdWRKY24 和 BdMYB22 相互作用。我们的研究结果为研究 BdHD1-转录因子复合物在 干旱胁迫反应中的作用提供了基础。
