Institute of Insect Science, Zhejiang University, Hangzhou 310058, China.
State Key Laboratory for Managing Biotic and Chemical Threats to the Quality and Safety of Agro-Products, Institute of Plant Virology, Ningbo University, Ningbo 315211, China.
Int J Mol Sci. 2020 Jun 13;21(12):4222. doi: 10.3390/ijms21124222.
gene belongs to the forkhead box gene family that comprises a diverse group of "winged helix" transcription factors that have been implicated in a variety of biochemical and cellular processes. In the brown planthopper (BPH), is highly expressed in the ovaries and newly laid eggs, where it acted as an indispensable gene through its molecular targets to regulate early embryonic development. Moreover, the results of the RNAi experiments indicated that () exhibited pleiotropism: they not only affected the embryogenesis, but also played an important role in molting. RNA-seq and RNAi were further used to reveal potential target genes of in different stages. In the eggs, ten downregulated genes were defined as potential target genes of because of the similar expression patterns and functions with . Knockdown of or any of these genes prevented the development of the eggs, resulting in a zero hatchability. In the nymphs, regulated the expression of a keratin gene, type I cytoskeletal keratin 9 (), to participate in the formation of an intermediate filament framework. Depletion of or in nymphs, BPHs failed to shed their old cuticle during nymph-to-nymph or nymph-to-adult molting and the mortality was almost 100%. Altogether, the pleiotropic roles of during embryogenesis and nymph molting were supported by the ability to coordinate the temporal and spatial gene expression of their target genes.
基因属于叉头框基因家族，该家族包含了多种“翼状螺旋”转录因子，这些转录因子参与了多种生化和细胞过程。在褐飞虱中，在卵巢和新产下的卵中高度表达，在那里它作为一个不可或缺的基因，通过其分子靶点来调节早期胚胎发育。此外，RNAi 实验的结果表明，在卵巢和新产下的卵中高度表达，在那里它作为一个不可或缺的基因，通过其分子靶点来调节早期胚胎发育。此外，RNAi 实验的结果表明， （）表现出多效性：它们不仅影响胚胎发生，而且在蜕皮中也起着重要作用。RNA-seq 和 RNAi 进一步用于揭示 不同阶段的潜在靶基因。在卵中，由于表达模式和功能与 相似，因此定义了十个下调基因作为 的潜在靶基因。敲低 或任何这些基因都会阻止卵的发育，导致孵化率为零。在若虫中， 调节角蛋白基因，I 型细胞骨架角蛋白 9 () 的表达，以参与中间丝框架的形成。在若虫中耗尽 或 ，褐飞虱在若虫蜕皮或若虫到成虫蜕皮期间无法蜕皮旧表皮，死亡率几乎为 100%。总之， 协调其靶基因的时空基因表达的能力支持了其在胚胎发生和若虫蜕皮中的多效性作用。
