Al Amin Md, Mahfujur Rahman Md, Razimi Mohd Shahril Ahmad, Chowdhury Zaira Zaman, Hussain Muhammad Nasri Md, Desa Mohd Nasir Mohd
Global Centre for Environmental Remediation (GCER), University of Newcastle, Callaghan, NSW, 2308, Australia.
Islamic Business School, Universiti Utara Malaysia, 06010, Kedah, Malaysia.
J Food Compost Anal. 2020 Sep;92:103565. doi: 10.1016/j.jfca.2020.103565. Epub 2020 Jun 9.
Determination of feline meat in food products is an important issue for social, health, economic and religious concern. Hence this paper documented the application of species specific polymerase chain reaction-restriction fragment length polymorphism (SP-PCR-RFLP) assay targeting a short-fragments (69 bp) of mitochondrial cytochrome () gene to screen feline meat in commercial meat products using lab-on-a-chip. The SP-PCR assay proved its specificity theoretically and experimentally while testing with different common animal, aquatic and plant species of DNA. The feline specific (69 bp, 43- and 26-bp) characteristic molecular DNA pattern was observed by SP-PCR and RFLP analysis. For assay performance, it was tested in three different types of commercial dummy meat products such as frankfurters, nuggets and meatballs and digested with I-restriction enzyme. The highest sensitivity of the assay using lab-on-a-chip was as low as 0.1 pg or 0.01 % (w/w) in commercial dummy meat products. We have also applied this assay to screen three important commercial meat products of six different brand from six supermarket chains located at three different states of Malaysia. Thus total 378 samples were tested to validate the specificity, sensitivity, stability of the assay and utilization of it for commercial meat product screening.
食品中猫肉的检测是一个涉及社会、健康、经济和宗教等多方面的重要问题。因此，本文记录了一种基于物种特异性聚合酶链反应-限制性片段长度多态性（SP-PCR-RFLP）分析方法的应用，该方法针对线粒体细胞色素（）基因的一个短片段（69 bp），利用芯片实验室技术筛选商业肉制品中的猫肉。在对不同常见动物、水生生物和植物物种的DNA进行检测时，SP-PCR分析在理论和实验上都证明了其特异性。通过SP-PCR和RFLP分析观察到了猫科动物特异性的（69 bp、43 bp和26 bp）特征性分子DNA模式。为了评估该分析方法的性能，在三种不同类型的商业模拟肉制品（如法兰克福香肠、鸡块和肉丸）中进行了测试，并用I型限制性内切酶进行消化。使用芯片实验室技术的该分析方法在商业模拟肉制品中的最高灵敏度低至0.1 pg或0.01%（w/w）。我们还应用该分析方法对来自马来西亚三个不同州的六个连锁超市的六个不同品牌的三种重要商业肉制品进行了筛选。因此，总共测试了378个样品，以验证该分析方法的特异性、灵敏度、稳定性及其在商业肉制品筛选中的实用性。
