Nischitha R, Vasanthkumari M M, Kumaraswamy B E, Shivanna M B
Department of PG Studies and Research in Applied Botany, Jnana Sahyadri, Kuvempu University, Shankaraghatta, Shimoga, 577 451 India.
Department of Entomology, University of Agricultural Science, GKVK, Banaglore, 560 065 India.
3 Biotech. 2020 Jul;10(7):300. doi: 10.1007/s13205-020-02250-0. Epub 2020 Jun 11.
There is a growing body of evidence that endophytic fungal metabolites possess important biological activities. (L.) Pers., a well-known grass species with potential medicinal properties, is under-explored for the diversity of endophytic fungal species and their metabolites. We report here the diversity of endophytic fungi in the culm, leaf and inflorescence of when cultured on moist blotter (MB), potato dextrose agar (PDA) and malt extract agar (MEA). Species richness, Shannon and Simpson diversity and evenness indices showed that PDA followed by MEA supported the growth of the largest number of fungal species. Amongst four fungal species tested, was selected for further studies on antimicrobial and antioxidant activities. The mycelial mat (MM) and culture filtrate (CF) of PD broth grown extracted with ethyl acetate and methanol, respectively, were subjected to antimicrobial assay against five bacterial and four fungal test isolates. Results indicated that the ethyl acetate extract of CF had moderate activity against and whereas the methanolic MM extract showed high to moderate activity to and . Cyclic voltammetric analysis of ethyl acetate extract showed very good antioxidant activity and the extract contained coumarins when determined by HPLC. High-resolution orbitrap LC-MS of ethyl acetate extract revealed the presence of metabolites with antimicrobial and antioxidant and other biological activities. Finding of the present study suggested that could be exploited for pharmaceutical applications.
越来越多的证据表明，内生真菌代谢产物具有重要的生物活性。黑麦草（Lolium perenne L.）是一种具有潜在药用特性的知名草种，但对于其内生真菌物种及其代谢产物的多样性研究较少。我们在此报告了黑麦草的茎、叶和花序中内生真菌在湿润滤纸（MB）、马铃薯葡萄糖琼脂（PDA）和麦芽提取物琼脂（MEA）上培养时的多样性。物种丰富度、香农和辛普森多样性及均匀度指数表明，PDA其次是MEA支持了最多数量真菌物种的生长。在所测试的四种真菌物种中，选择了一种进行抗菌和抗氧化活性的进一步研究。分别用乙酸乙酯和甲醇提取在PD肉汤中生长的该真菌的菌丝体垫（MM）和培养滤液（CF），对五种细菌和四种真菌测试分离株进行抗菌测定。结果表明，CF的乙酸乙酯提取物对两种细菌有中等活性，而甲醇MM提取物对三种细菌表现出高到中等活性。乙酸乙酯提取物的循环伏安分析显示出非常好的抗氧化活性，通过HPLC测定时提取物含有香豆素。乙酸乙酯提取物的高分辨率轨道阱液相色谱 - 质谱显示存在具有抗菌、抗氧化和其他生物活性的代谢产物。本研究结果表明，黑麦草可用于制药应用。
