Singh Tej, Jyoti Kumari, Patnaik Amar, Singh Ajeet, Chauhan Ranchan, Chandel S S
Department of Mechanical Engineering, Manav Bharti University, Solan 173229, India.
Department of Biotechnology, G.B.P.E.C., Pauri Garhwal, 246194, India.
J Genet Eng Biotechnol. 2017 Jun;15(1):31-39. doi: 10.1016/j.jgeb.2017.04.005. Epub 2017 Apr 22.
In this study, biological synthesis of silver nanoparticles (AgNPs) from supernatant of endophytic fungus sp. isolated from the healthy leaves of is studied. The synthesized AgNPs are characterized using UV-vis spectroscopy and Fourier transform-infrared spectroscopy (FTIR). The structural analysis is done by powder X-ray diffraction (XRD) method. The stability of AgNPs is studied by dynamic light scattering (DLS) method. The size and shape of AgNPs are observed by transmission electron microscopy (TEM) and atomic force microscopy (AFM) and found to be spherical with an average particles size of 4-30 nm. Further, these AgNPs have been found to be highly toxic against human pathogenic bacteria, suggesting the possibility of using AgNPs as efficient antibacterial agents.
在本研究中，对从[植物名称]健康叶片中分离出的内生真菌菌株的上清液进行了银纳米颗粒（AgNPs）的生物合成研究。使用紫外可见光谱和傅里叶变换红外光谱（FTIR）对合成的AgNPs进行了表征。通过粉末X射线衍射（XRD）方法进行结构分析。通过动态光散射（DLS）方法研究了AgNPs的稳定性。通过透射电子显微镜（TEM）和原子力显微镜（AFM）观察了AgNPs的尺寸和形状，发现其为球形，平均粒径为4 - 30纳米。此外，已发现这些AgNPs对人类致病细菌具有高毒性，这表明使用AgNPs作为高效抗菌剂的可能性。
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