Zhang Di, Lv Aimin, Yang Tianchen, Cheng Xiaoqing, Zhao Enhua, Zhou Peng
School of Agriculture and Biology, Shanghai Jiao Tong University, Shanghai 200240, China.
School of Design, Shanghai Jiao Tong University, Shanghai 200240, China.
Gene X. 2020 Apr 22;5:100033. doi: 10.1016/j.gene.2020.100033. eCollection 2020 Dec.
Dehydrins (DHNs) play critical roles in plant adaptation to abiotic stresses. The objective of this study was to characterize DHNs in bermudagrass ( spp.). gene was cloned from bermudagrass 'Tifway'. Two transcripts were detected due to alternative splicing (the nonspliced - and the spliced -) and both the CdDHN4-S and CdDHN4-L proteins are YSK-type DHNs, the Φ-segment is present in CdDHN4-L and absent in CdDHN4-S. Transgenic expressing - or - exhibited improved tolerance to salt, osmotic, low temperature and drought stress compared to the wild type (WT). The two transgenic lines did not differ in salt or drought tolerance, while plants expressing - grew better under osmotic stress than those expressing -. Both transgenic lines exhibited reduced content of malondialdehyde (MDA) and reactive oxygen species (ROS); and higher antioxidant enzymatic activities than the wild type plants under salt or drought stress. - exhibited a higher ROS-scavenging capacity than -.
脱水素（DHNs）在植物适应非生物胁迫过程中发挥着关键作用。本研究的目的是鉴定狗牙根（ spp.）中的脱水素。从狗牙根‘Tifway’中克隆出 基因。由于可变剪接检测到两种 转录本（未剪接的 - 和剪接的 -），并且CdDHN4-S和CdDHN4-L蛋白均为YSK型脱水素，Φ片段存在于CdDHN4-L中而不存在于CdDHN4-S中。与野生型（WT）相比，表达 - 或 - 的转基因植株对盐、渗透、低温和干旱胁迫的耐受性有所提高。这两个转基因株系在耐盐性或耐旱性方面没有差异，而表达 - 的植株在渗透胁迫下比表达 - 的植株生长得更好。在盐胁迫或干旱胁迫下，两个转基因株系的丙二醛（MDA）和活性氧（ROS）含量均降低；且抗氧化酶活性均高于野生型植株。 - 表现出比 - 更高的ROS清除能力。
