School of Agriculture and Biology, Shanghai Jiao Tong University, Shanghai 200240, China; School of Design, Shanghai Jiao Tong University, Shanghai 200240, China.
School of Agriculture and Biology, Shanghai Jiao Tong University, Shanghai 200240, China.
Gene. 2020 Dec;763S:100033. doi: 10.1016/j.gene.2020.100033. Epub 2020 Apr 22.
Dehydrins (DHNs) play critical roles in plant adaptation to abiotic stresses. The objective of this study was to characterize DHNs in bermudagrass (Cynodon spp.). CdDHN4 gene was cloned from bermudagrass 'Tifway'. Two CdDHN4 transcripts were detected due to alternative splicing (the nonspliced CdDHN4-L and the spliced CdDHN4-S) and both the CdDHN4-S and CdDHN4-L proteins are YSK-type DHNs, the Φ-segment is present in CdDHN4-L and absent in CdDHN4-S. Transgenic Arabidopsis thaliana expressing CdDHN4-L or CdDHN4-S exhibited improved tolerance to salt, osmotic, low temperature and drought stress compared to the wild type (WT). The two transgenic lines did not differ in salt or drought tolerance, while plants expressing CdDHN4-S grew better under osmotic stress than those expressing CdDHN4-L. Both transgenic lines exhibited reduced content of malondialdehyde (MDA) and reactive oxygen species (ROS); and higher antioxidant enzymatic activities than the wild type plants under salt or drought stress. CdDHN4-S exhibited a higher ROS-scavenging capacity than CdDHN4-L.
脱水素（DHNs）在植物适应非生物胁迫中发挥着关键作用。本研究旨在鉴定百慕大草（Cynodon spp.）中的 DHNs。从百慕大草 'Tifway' 中克隆出 CdDHN4 基因。由于选择性剪接，检测到两种 CdDHN4 转录本（未剪接的 CdDHN4-L 和剪接的 CdDHN4-S），CdDHN4-S 和 CdDHN4-L 蛋白均为 YSK 型 DHN，Φ 片段存在于 CdDHN4-L 中，不存在于 CdDHN4-S 中。与野生型（WT）相比，表达 CdDHN4-L 或 CdDHN4-S 的转基因拟南芥对盐、渗透、低温和干旱胁迫的耐受性提高。这两个转基因系在耐盐性或耐旱性方面没有差异，而表达 CdDHN4-S 的植物在渗透胁迫下的生长状况优于表达 CdDHN4-L 的植物。在盐或干旱胁迫下，与野生型植物相比，这两个转基因系的丙二醛（MDA）和活性氧（ROS）含量降低，抗氧化酶活性升高。CdDHN4-S 比 CdDHN4-L 具有更高的 ROS 清除能力。
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