Gutiérrez-Santa Ana Andrea, Carrillo-Cerda H A, Rodriguez-Campos J, Kirchmayr M R, Contreras-Ramos S M, Velázquez-Fernández J B
Unidad de Tecnología Ambiental, Centro de Investigación y Asistencia en Tecnología y Diseño del Estado de Jalisco A.C. (CIATEJ), Av. Normalistas No. 800, Col. Colinas de la Normal, 44270 Guadalajara, Jalisco Mexico.
Unidad de Servicios Analíticos y Metrológicos, CIATEJ, Av. Normalistas No. 800, Col. Colinas de la Normal, 44270 Guadalajara, Mexico.
3 Biotech. 2020 Jul;10(7):292. doi: 10.1007/s13205-020-02290-6. Epub 2020 Jun 6.
The aims of this work were to screen isolated bacteria with a dual capacity: to inhibit and to promote plant growth. Also, volatile compounds that would be responsible for that effect were identified. Seventy bacterial strains from the air, agricultural soils, hydrocarbons-contaminated soils, and extremophile soils were tested. The former were identified by Matrix-Assisted Laser Desorption/Ionization-time of flight mass spectrometry and 16S rDNA sequencing. The plant growth-promoting bacteria (PGPB) and their capability for phosphate solubilization, siderophores production, and indole production were determined. Twenty isolates from and genera inhibited the mycelial growth up to 40% in direct assays. Eleven isolates significantly inhibited mycelial growth in 18-24% via volatile emissions. Volatile compounds related to antifungal activity or stress response include ketones, sesquiterpenes, monoterpenoids, alkanes, and fatty acids. Our results support the potential of these PGPB to act as biocontrol agents against fungal pathogens via volatile emissions.
抑制和促进植物生长。此外，还鉴定了造成这种效果的挥发性化合物。对来自空气、农业土壤、碳氢化合物污染土壤和极端嗜热土壤的70株细菌菌株进行了测试。前者通过基质辅助激光解吸/电离飞行时间质谱和16S rDNA测序进行鉴定。测定了植物促生细菌（PGPB）及其溶解磷酸盐、产生铁载体和吲哚的能力。在直接测定中，来自[具体属名]和[具体属名]的20株分离物抑制菌丝体生长高达40%。11株分离物通过挥发性排放显著抑制18%-24%的菌丝体生长。与抗真菌活性或应激反应相关的挥发性化合物包括酮类、倍半萜类、单萜类、烷烃和脂肪酸。我们的结果支持了这些PGPB通过挥发性排放作为真菌病原体生物防治剂的潜力。
