Abou Baker Doha H, Ibrahim Bassant M M, Hassan Nabila S, Yousuf A F, Gengaihi Souad El
Medicinal and Aromatic Plants Department. National Research Centre, Dokki, Giza. PO 12622, Egypt.
Pharmacology Department. Medical Research Division. National Research Centre, Dokki, Giza, PO 12622, Egypt.
Toxicol Rep. 2020 Jun 5;7:723-729. doi: 10.1016/j.toxrep.2020.06.001. eCollection 2020.
Fruit by-products are considered nature's golden gift for human health and a good starting point to discover new drugs depending on the fact that they contain millions of bio-active compounds that are responsible for therapeutic activities. In this context, the main goal of this study is to recycle () seeds to produce pharmaceutical molecules to be used in the prevention of the progressive neurological damage associated with Alzheimer disease (AD). Donepezil (0.75 mg/kg), hesperidin (125 and 250 mg/kg) and limonoids (50 and 100 mg/kg) were used for treatment of rats for 2 weeks prior to concomitant administration of AlCl for three successive weeks. Protection against cognitive deterioration was observed among study group with insignificant difference from normal control group and significant difference from positive control group in the Y-Maze test. On the other hand, treatment with both doses of hesperidin (125 and 250 mg/kg) and high dose of limonoids only (100 mg/kg) produced improvement in psychological state, observed by significant increase in ambulation frequency in comparison to positive control group, however it was not as frequent as normal group, as it was significantly less than normal group in the open field test. Regarding acetylcholine esterase (AChE) and beta-amyloid (β amyloid) levels, the effect of limonoids low dose was the best as it didn't have a significant effect when compared to normal control, also hesperidin in both doses showed insignificant effects on β amyloid levels when compared to normal control group. Our results encourage the use of seeds which are wasted in huge amounts, as Alzheimer prophylactic food additives.
水果副产品被认为是大自然赐予人类健康的珍贵礼物，也是发现新药的良好起点，因为它们含有数百万种具有治疗活性的生物活性化合物。在此背景下，本研究的主要目标是回收（）种子，以生产用于预防与阿尔茨海默病（AD）相关的进行性神经损伤的药物分子。在连续三周给予氯化铝之前，先用多奈哌齐（0.75毫克/千克）、橙皮苷（125和250毫克/千克）和柠檬苦素（50和100毫克/千克）对大鼠进行为期2周的治疗。在Y迷宫试验中，研究组观察到对认知衰退有保护作用，与正常对照组无显著差异，与阳性对照组有显著差异。另一方面，两种剂量的橙皮苷（125和250毫克/千克）和仅高剂量的柠檬苦素（100毫克/千克）治疗均使心理状态有所改善，与阳性对照组相比，行走频率显著增加，但不如正常组频繁，因为在旷场试验中显著低于正常组。关于乙酰胆碱酯酶（AChE）和β淀粉样蛋白（β-淀粉样蛋白）水平，低剂量柠檬苦素的效果最佳，与正常对照组相比无显著影响，与正常对照组相比，两种剂量的橙皮苷对β淀粉样蛋白水平也无显著影响。我们的研究结果鼓励将大量被浪费的（）种子用作阿尔茨海默病预防性食品添加剂。
