HPLC-DAD指纹图谱分析、（某种植物，原文未完整写出学名）叶的抗氧化活性及其对与阿尔茨海默病相关关键酶的抑制作用
HPLC-DAD fingerprinting analysis, antioxidant activities of (Hemsl.) A. Gray leaves and its inhibition of key enzymes linked to Alzheimer's disease.
作者信息
Ojo Oluwafemi Adeleke, Ojo Adebola Busola, Ajiboye Basiru Olaitan, Olaiya Oluranti, Okesola Mary Abiola, Boligon Aline Augusti, de Campos Marli Matiko Anraku, Oyinloye Babatunji Emmanuel, Kappo Abidemi Paul
机构信息
Phytomedicine and Biochemical Toxicology Unit, Department of Biochemistry, Afe Babalola University, Ado-Ekiti, Mail Bag, 5454, Nigeria.
Department of Biochemistry, University of Ilorin, Ilorin, Nigeria.
出版信息
Toxicol Rep. 2018 May 15;5:585-592. doi: 10.1016/j.toxrep.2018.05.003. eCollection 2018.
(Hemsl.) A. Gray leaves have long been used to manage neurodegenerative diseases without scientific basis. This study characterized the phenolic constituents, evaluated the antioxidant properties of phenolic extracts from leaves used as traditional medicine in Africa and its inhibition of key enzymes linked to Alzheimer's disease. The extract was rich in phenolic acids (gallic acid, chlorogenic acid, caffeic acid and -coumaric acid) and flavonoids (apigenin) and had 1,1-diphenyl-2-picryl-hydrazil radical scavenging abilities (IC = 41.05 μg. mL), 2,2-Azino-bis3-ethylbenthiazoline-6sulphonic acid radical scavenging ability (IC = 33.51 μg. mL), iron chelation (IC = 38.50 μg. mL), reducing power (Fe- Fe) (7.34 AAEmg/100 g), inhibited acetylcholinesterase (IC = 39.27 μg mL) and butyrylcholinesterase (IC = 35.01 μg mL) activities. These results reveal the leaf as a rich source of phenolic compounds with antioxidant and cholinesterase inhibitory activity.
（赫姆斯利）A. 格雷叶长期以来一直被用于治疗神经退行性疾病，但缺乏科学依据。本研究对酚类成分进行了表征，评估了非洲传统药用叶中酚类提取物的抗氧化性能及其对与阿尔茨海默病相关的关键酶的抑制作用。该提取物富含酚酸（没食子酸、绿原酸、咖啡酸和对香豆酸）和黄酮类化合物（芹菜素），具有1,1-二苯基-2-苦基肼自由基清除能力（IC₅₀ = 41.05 μg·mL）、2,2-联氮-双-（3-乙基苯并噻唑啉-6-磺酸）自由基清除能力（IC₅₀ = 33.51 μg·mL）、铁螯合能力（IC₅₀ = 38.50 μg·mL）、还原能力（Fe²⁺-Fe³⁺）（7.34 AAEmg/100 g），抑制乙酰胆碱酯酶（IC₅₀ = 39.27 μg·mL）和丁酰胆碱酯酶（IC₅₀ = 35.01 μg·mL）的活性。这些结果表明该叶是具有抗氧化和胆碱酯酶抑制活性的酚类化合物的丰富来源。
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