Zietchick Research Institute (ZRI), Plymouth, MI, USA.
Department of Pediatrics and Human Development, College of Human Medicine, Michigan State University, East Lansing, MI, USA.
Biomarkers. 2020 Sep;25(6):468-473. doi: 10.1080/1354750X.2020.1783573. Epub 2020 Jun 28.
Retinopathy of prematurity (ROP) is a potentially serious eye disorder affecting very preterm infants. Non-proliferative ROP (NP-ROP), also known as Early Stage ROP, is characterized by deficient retinal angiogenesis. Proliferative ROP (P-ROP), also known as Late Stage ROP, is characterized by pathologic angiogenesis. The use of neonatal haemoglobin A1C as a biomarker for ROP has not yet been evaluated.
We modified the Haemoglobin A1C assay for use with neonatal dried blood spots (DBS) and then assessed A1C levels via Elisa immunoassay on DBS from 43 preterm infants (with gestational ages 26-28weeks). We measured A1C on DBS collected at <1week and 4weeks of chronological age.
Compared to matched counterparts without ROP, there is significantly lower HbA1c in infants who develop NP-ROP, this occurs at Week 4 (0.004), but is not seen at Week 1; there is significantly higher HbA1c in infants with P-ROP, this occurs both at Week 1 (0.05) and Week 4 (0.005).
The A1C test, modified for use with DBS, is a feasible biomarker for ROP; low A1C is a potential biomarker for non-proliferative ROP and high A1C is for proliferative ROP.
早产儿视网膜病变（ROP）是一种可能影响极早产儿的严重眼部疾病。非增生性 ROP（NP-ROP），又称早期 ROP，其特征是视网膜血管生成不足。增生性 ROP（P-ROP），又称晚期 ROP，其特征是病理性血管生成。尚未评估新生儿血红蛋白 A1C 作为 ROP 的生物标志物。
我们修改了用于新生儿干血斑（DBS）的血红蛋白 A1C 测定法，然后通过 DBS 上的 ELISA 免疫分析法评估了 43 名早产儿（胎龄 26-28 周）的 A1C 水平。我们在 1 周和 4 周的生理年龄时测量 DBS 上的 A1C。
与无 ROP 的匹配对照相比，发生 NP-ROP 的婴儿的 HbA1c 明显较低，这发生在第 4 周（0.004），但在第 1 周没有；患有 P-ROP 的婴儿的 HbA1c 明显较高，这发生在第 1 周（0.05）和第 4 周（0.005）。
用于 DBS 的 A1C 测试是 ROP 的可行生物标志物；低 A1C 是 NP-ROP 的潜在生物标志物，高 A1C 是 P-ROP 的生物标志物。
