College of Life science/State Key Laboratory of Crop Stress Biology for Arid Areas, Northwest A&F University , Yangling, Shannxi, China.
College of Nursing, Weinan Vocational&Technical College , Weinan, Shannxi, China.
Plant Signal Behav. 2020 Aug 2;15(8):1778920. doi: 10.1080/15592324.2020.1778920. Epub 2020 Jun 19.
As stress-inducible proteins, dehydrins exert functional protective role by alleviating the cell damage when plants are suffering from the stresses. However, the upstream regulatory mechanism of these proteins is not very clear. To unravel the regulatory mechanism of dehydrin, a screen of wheat cDNA library from cold and PEG-treated wheat seedlings was performed and a transcription factor TaERF4a (GenBank NO. AFP49822.1) interacting with wheat dehydrin gene (Gene ID: 100037544) promoter was identified by yeast one-hybrid assay. The regulator and the gene can respond to the abiotic stress, the induced transcripts of these two genes exhibit a similar expression trend under adverse environmental conditions. In planta, the dual luciferase transient assay analysis showed that TaERF4a can positively regulate the expression of WZY1-2 dehydrin. Therefore, the obtained results suggest that ERF transcription factor can regulate the expression level of the dehydrin gene in wheat.
作为应激诱导蛋白，脱水素在植物受到胁迫时通过减轻细胞损伤发挥功能保护作用。然而，这些蛋白质的上游调控机制尚不清楚。为了阐明脱水素的调控机制，我们通过酵母单杂交试验从冷胁迫和 PEG 处理的小麦幼苗 cDNA 文库中筛选到一个与小麦脱水素基因（基因 ID：100037544）启动子相互作用的转录因子 TaERF4a（GenBank NO. AFP49822.1）。该调节剂和基因都能响应非生物胁迫，在不利的环境条件下，这两个基因的诱导转录本表现出相似的表达趋势。在体内，双荧光素酶瞬时测定分析表明 TaERF4a 可以正向调节 WZY1-2 脱水素的表达。因此，研究结果表明 ERF 转录因子可以调节小麦脱水素基因的表达水平。
