K-/S- 段对小麦脱水素 WZY1-2 亚细胞定位和二聚化的影响。
Effect of K-/S- segments on subcellular localization and dimerization of wheat dehydrin WZY1-2.
机构信息
College of Life Science, Northwest A & F University , Yangling, Shaanxi, PR China.
出版信息
Plant Signal Behav. 2020 Dec 1;15(12):1827583. doi: 10.1080/15592324.2020.1827583. Epub 2020 Oct 5.
Dehydrins (DHNs) belong to group Ⅱ late embryogenesis abundant (LEA) proteins which perform multiple functions in plants during stress conditions. Both K- and S-segments are conserved domains in the dehydrin protein family; however, there are only a few functional studies for these two conserved segments. In this study, the DHN gene was isolated from and its K-/S-segment-truncated derivatives were generated. In order to explore the biological function of these two conserved fragments, subcellular localization and dimerization detection assays were performed for the K-/S-segment-truncated derivatives. Results of GFP fusion and bimolecular fluorescence complementation (BiFC) assays indicated that WZY1-2 localized to nucleus as a homologous dimer. The S-segment partially regulated the nuclear localization of WZY1-2 but did not affect its dimerization, while the K-segment influenced neither the dimer formation nor the subcellular localization.
脱水素（DHN）属于第Ⅱ组晚期胚胎丰富（LEA）蛋白，在植物胁迫条件下发挥多种功能。K-和 S-片段都是脱水素蛋白家族中的保守结构域；然而，对于这两个保守片段的功能研究很少。在这项研究中，从 中分离出 DHN 基因，并生成其 K-/S-片段截断衍生物。为了探索这两个保守片段的生物学功能，对 K-/S-片段截断衍生物进行了亚细胞定位和二聚化检测实验。GFP 融合和双分子荧光互补（BiFC）实验的结果表明，WZY1-2 作为同源二聚体定位于细胞核。S-片段部分调节 WZY1-2 的核定位，但不影响其二聚化，而 K-片段既不影响二聚体形成，也不影响亚细胞定位。
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