CEB - Centre of Biological Engineering, University of Minho, Campus of Gualtar, 4710-057, Braga, Portugal.
CBMA - Centre of Molecular and Environmental Biology, University of Minho, Campus of Gualtar, 4710-057, Braga, Portugal.
Nanomedicine (Lond). 2020 Jun;15(15):1459-1469. doi: 10.2217/nnm-2020-0089. Epub 2020 Jun 17.
Alopecia treatments are scarce and lack efficacy. Cyclosporin A (CsA) has hair growth-inducing properties but its poor cutaneous absorption undermines its use in topical treatments. Development of a new potential topical treatment of alopecia with CsA. CsA-loaded poly(d,l-lactide) (PLA) nanoparticles were obtained and characterized. Skin permeation was evaluated in porcine skin. Nanoparticles with good physicochemical stability increased CsA skin permeation/hair follicles accumulation, compared with a noncolloidal formulation. CsA biocompatibility in NCTC2455 keratinocytes (reference skin cell line) was clearly improved when encapsulated in PLA nanoparticles. This work fosters further investigation of CsA-loaded PLA nanoparticles as a promising new strategy to treat alopecia, a very traumatic, possibly autoimmune, disease.
脱发治疗方法稀缺且疗效不佳。环孢素 A（CsA）具有促进头发生长的特性，但由于其皮肤吸收不良，限制了其在局部治疗中的应用。 开发一种新的潜在脱发局部治疗方法，用 CsA。 负载环孢素 A 的聚（D，L-丙交酯）（PLA）纳米粒被获得并进行了表征。 在猪皮上评估了皮肤渗透。 与非胶体制剂相比，具有良好理化稳定性的纳米粒增加了 CsA 的皮肤渗透/毛囊蓄积。 当 CsA 被包裹在 PLA 纳米粒中时，其在 NCTC2455 角质细胞（参考皮肤细胞系）中的生物相容性明显改善。 这项工作促进了负载 CsA 的 PLA 纳米粒作为一种有前途的新策略的进一步研究，用于治疗脱发，这是一种非常创伤性的、可能是自身免疫性的疾病。
