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高盐洗涤的大肠杆菌核糖体中核糖核酸酶II的存在:对核糖体复合物圆二色性的影响。

The presence of RNase II in high salt washed E. coli ribosomes: effect on circular dichroism of ribosomal complexes.

作者信息

Butzow J J, Eichhorn G L

出版信息

Nucleic Acids Res. 1977 Apr;4(4):867-76. doi: 10.1093/nar/4.4.867.

Abstract

Traces of the exonuclease RNAse II are retained by E. coli ribosomes purified by conventional high salt washing. The residual activity can give rise to serious artifacts, especially in physical studies.

摘要

通过常规高盐洗涤纯化的大肠杆菌核糖体保留了核酸外切酶RNAse II的痕迹。残留活性可能会导致严重的假象,尤其是在物理研究中。

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