大肠杆菌核糖核酸酶V。I. 对核糖体和转位的依赖性。
Ribonuclease V of escherichia coli. I. Dependence on ribosomes and translocation.
作者信息
Kuwano M, Kwan C N, Apirion D, Schlessinger D
出版信息
Proc Natl Acad Sci U S A. 1969 Oct;64(2):693-700. doi: 10.1073/pnas.64.2.693.
Abstract
A new RNase activity, tentatively named RNase V, was found in cell-free extracts of E. coli. This activity requires ribosomes, G and T factors, tRNA, K(+) or NH(4) (+), Mg(2+), GTP, and a sulfhydryl compound to degrade poly U, poly A, T4 phage mRNA, or E. coli mRNA. RNase V is specific for mRNA; it does not attack ribosomal RNA. It is inhibited by antibiotics that decrease breakdown of mRNA in vivo, such as chloramphenicol and streptomycin, and by such agents as 5'-beta, gamma-methylene-guanosine triphosphate, and fusidic acid, which inhibit ribosome-dependent GTPase and translocation of ribosomes along mRNA. The evidence suggests that RNase V is either an integral part of the ribosome or is tightly associated with it, and that it selectively degrades mRNA in intact cells.
摘要
在大肠杆菌的无细胞提取物中发现了一种新的核糖核酸酶活性，暂命名为核糖核酸酶V。这种活性需要核糖体、G因子和T因子、转运RNA、钾离子或铵离子、镁离子、鸟苷三磷酸以及一种巯基化合物来降解多聚尿苷酸、多聚腺苷酸、T4噬菌体信使核糖核酸或大肠杆菌信使核糖核酸。核糖核酸酶V对信使核糖核酸具有特异性；它不攻击核糖体核糖核酸。它会受到体内能减少信使核糖核酸分解的抗生素（如氯霉素和链霉素）以及能抑制核糖体依赖性鸟苷三磷酸酶和核糖体沿信使核糖核酸移位的试剂（如5'-β，γ-亚甲基鸟苷三磷酸和夫西地酸）的抑制。证据表明，核糖核酸酶V要么是核糖体的一个组成部分，要么与之紧密相关，并且它在完整细胞中选择性地降解信使核糖核酸。
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本文引用的文献
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AMINO ACID INCORPORATION INTO PROTEINS BY ESCHERICHIA COLI RIBOSOMES.大肠杆菌核糖体将氨基酸掺入蛋白质的过程。
Proc Natl Acad Sci U S A. 1960 Nov;46(11):1450-63. doi: 10.1073/pnas.46.11.1450.
2
Hydrolysis of oligoribonucleotides by an enzyme fraction from Escherichia coli.大肠杆菌中一种酶组分对寡核糖核苷酸的水解作用。
Biochem Biophys Res Commun. 1967 Nov 30;29(4):550-5. doi: 10.1016/0006-291x(67)90520-7.
3
Characteristics and stabilization of DNAase-sensitive protein synthesis in E. coli extracts.大肠杆菌提取物中脱氧核糖核酸酶敏感蛋白合成的特性与稳定性
Proc Natl Acad Sci U S A. 1961 Oct 15;47(10):1580-8. doi: 10.1073/pnas.47.10.1580.
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Messenger RNA turnover and protein synthesis in B. subtilis inhibited by actinomycin D.枯草芽孢杆菌中受放线菌素D抑制的信使核糖核酸周转和蛋白质合成
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J Biol Chem. 1964 Nov;239:3716-26.
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