Department of Brain and Cognitive Sciences, Daegu Gyeongbuk Institute of Science and Technology (DGIST), 333 Techno Jungangdae-Ro, Hyeonpoong-Eup, Dalseong-Gun, Daegu, 42988, South Korea.
Core Protein Resources Center, DGIST, 333 Techno Jungangdae-Ro, Hyeonpoong-Eup, Dalseong-Gun, Daegu, 42988, South Korea.
Mol Brain. 2020 Jun 17;13(1):94. doi: 10.1186/s13041-020-00629-x.
Members of the leukocyte common antigen-related receptor protein tyrosine phosphatase (LAR-RPTP) family, comprising PTPσ, PTPδ and LAR, are key hubs for presynaptic assembly and differentiation in vertebrate neurons. However, roles of individual LAR-RPTP members have not been investigated using member-specific conditional knockout mice. Here, we show that loss of PTPδ had no overt effect on synapse development in mouse cultured hippocampal neurons. Moreover, loss of PTPδ in presynaptic CA1 hippocampal neurons did not influence neurotransmitter release in subicular pyramidal neurons, suggesting that PTPδ is not critical for presynaptic function in vivo. Our results demonstrate that PTPδ is not essential for synapse maintenance or transmission, at least in the mouse hippocampus, and underscore the importance of using sophisticated genetic approaches to confirm the roles of synaptic proteins.
白细胞共同抗原相关受体蛋白酪氨酸磷酸酶（LAR-RPTP）家族成员包括 PTPσ、PTPδ 和 LAR，是脊椎动物神经元中突触前装配和分化的关键枢纽。然而，利用特定于成员的条件性敲除小鼠，尚未研究单个 LAR-RPTP 成员的作用。在这里，我们发现 PTPδ 的缺失对培养的海马神经元中的突触发育没有明显影响。此外，PTPδ 在 CA1 海马神经元中的缺失并不影响下丘椎体神经元中的神经递质释放，表明 PTPδ 对于体内的突触前功能不是必需的。我们的结果表明，PTPδ 对于突触维持或传递不是必需的，至少在小鼠海马体中是这样，这突显了使用复杂的遗传方法来确认突触蛋白作用的重要性。
