Senova Gesellschaft für Biowissenschaft und Technik mbH, Industriestraße 8, 99427, Weimar, Germany.
Senova Gesellschaft für Biowissenschaft und Technik mbH, Industriestraße 8, 99427, Weimar, Germany.
Biosens Bioelectron. 2020 Sep 15;164:112324. doi: 10.1016/j.bios.2020.112324. Epub 2020 May 30.
Antibody-modified gold nanomaterials are central to many novel biosensing technologies for example the lateral flow assays technology. The combination of the specificity, provided by antibody-antigen interactions, and the unique optical properties of nanomaterials provide excellent properties for biosensors. Here, we present the use of gold nanorods (GNR) with the localized Surface Plasmon Resonance (LSPR) peak in the visible range for biomarker detection. The colour of the GNR can be tuned by the reaction conditions to provide multi-coloured gold nanorod conjugates. These antibody functionalized GNR have the potential to provide significant improvements in multiplexed analysis and sensitivity compared to conventional gold nanoparticle based lateral flow assays. However, a major challenge is the synthesis of stable conjugates that resist aggregation in samples with high ionic strength, (e.g. salt solutions) and allow highly sensitive detection of proteins. A detailed investigation of different reagents for the functionalization of gold nanorod materials are reported. An antibody modified GNR based lateral flow assay is validated for the determination of C-reactive Protein (CRP).
抗体修饰的金纳米材料是许多新型生物传感技术的核心，例如侧向流动分析技术。抗体-抗原相互作用提供的特异性与纳米材料的独特光学特性相结合，为生物传感器提供了卓越的性能。在这里，我们展示了使用具有局域表面等离子体共振（LSPR）峰在可见范围内的金纳米棒（GNR）进行生物标志物检测。通过反应条件可以调整 GNR 的颜色，以提供多色金纳米棒缀合物。与传统基于金纳米粒子的侧向流动分析相比，这些抗体功能化的 GNR 有可能在多重分析和灵敏度方面提供显著的改进。然而，一个主要的挑战是合成稳定的缀合物，这些缀合物能够抵抗高离子强度样品中的聚集（例如盐溶液），并允许对蛋白质进行高灵敏度的检测。本文详细研究了不同的试剂用于金纳米棒材料的功能化。基于抗体修饰的 GNR 的侧向流动分析已被验证用于 C-反应蛋白（CRP）的测定。
