Laboratoire de Réactivité de Surface (LRS), Sorbonne Université, CNRS, UMR 7197, 4 Place Jussieu, F-75005 Paris, France.
Institut Parisien de Chimie Moléculaire (IPCM), Sorbonne Université, CNRS, 4 Place Jussieu, F-75005 Paris, France.
Biosensors (Basel). 2020 Oct 17;10(10):146. doi: 10.3390/bios10100146.
Nanoparticles made of coinage metals are well known to display unique optical properties stemming from the localized surface plasmon resonance (LSPR) phenomenon, allowing their use as transducers in various biosensing configurations. While most of the reports initially dealt with spherical gold nanoparticles owing to their ease of synthesis, the interest in gold nanorods (AuNR) as plasmonic biosensors is rising steadily. These anisotropic nanoparticles exhibit, on top of the LSPR band in the blue range common with spherical nanoparticles, a longitudinal LSPR band, in all respects superior, and in particular in terms of sensitivity to the surrounding media and LSPR-biosensing. However, AuNRs synthesis and their further functionalization are less straightforward and require thorough processing. In this paper, we intend to give an up-to-date overview of gold nanorods in LSPR biosensing, starting from a critical review of the recent findings on AuNR synthesis and the main challenges related to it. We further highlight the various strategies set up to coat AuNR with a silica shell of controlled thickness and porosity compatible with LSPR-biosensing. Then, we provide a survey of the methods employed to attach various bioreceptors to AuNR. Finally, the most representative examples of AuNR-based LSPR biosensors are reviewed with a focus put on their analytical performances.
基于局域表面等离子体共振（LSPR）现象，硬币金属纳米粒子具有独特的光学性质，可将其用作各种生物传感配置中的换能器。虽然由于其易于合成，最初大多数报道都涉及球形金纳米粒子，但对金纳米棒（AuNR）作为等离子体生物传感器的兴趣正在稳步上升。这些各向异性纳米粒子除了具有与球形纳米粒子共同的蓝色范围内的 LSPR 带之外，还具有纵向 LSPR 带，在各方面都更优越，尤其是在对周围介质和 LSPR 生物传感的灵敏度方面。然而，AuNR 的合成及其进一步功能化并不那么简单，需要进行彻底的处理。在本文中，我们打算从 AuNR 合成的最新研究成果和与之相关的主要挑战的批判性回顾开始，对 LSPR 生物传感中的金纳米棒进行最新的概述。我们进一步强调了各种策略，这些策略旨在将具有可控厚度和与 LSPR 生物传感兼容的多孔性的二氧化硅壳涂覆到 AuNR 上。然后，我们提供了用于将各种生物受体连接到 AuNR 的方法的调查。最后，对基于 AuNR 的 LSPR 生物传感器的最具代表性的示例进行了综述，重点介绍了它们的分析性能。
