白细胞介素 12A 和白细胞介素 12B 基因变异与突尼斯慢性乙型肝炎感染的相互作用分析。
Interaction analysis of IL-12A and IL-12B gene variants with chronic hepatitis B infection in Tunisian patients.
机构信息
Laboratory of Microbiology, Genetic Characterization of Infectious Diseases, UR12SP34 University Hospital Farhat Hached, Sousse, Tunisia; Laboratory of Studying Biological and Genetic Markers for Early Diagnosis and Follow-Up of Neurological Diseases, LR18ES47, Faculty of Medicine, Sousse, Tunisia; High Institute of Applied Sciences and Technology, Mahdia, Tunisia.
Laboratory of Microbiology, Genetic Characterization of Infectious Diseases, UR12SP34 University Hospital Farhat Hached, Sousse, Tunisia.
出版信息
Immunol Lett. 2020 Sep;225:50-56. doi: 10.1016/j.imlet.2020.06.004. Epub 2020 Jun 15.
Given the key role of interleukin-12 (IL-12) in the control of HBV, we investigated the possible correlation between IL-12A rs568408 and IL-12B rs3212227 polymorphisms and the risk of chronic HBV infection in Tunisian population. Two hundred patients with chronic HBV infection and two hundred healthy controls were genotyped using PCR-RFLP. A allele, AA and AG genotypes of IL-12A rs568408 were more represented in the chronic HBV infection group compared to the control group, and they were associated with 1.65-, 2.58- and 3.13-fold risks of developing this infection, respectively. Gene-gene interaction analysis showed that subjects carrying the IL-12A rs568408AA/AG and IL-12B rs3212227AA genotypes had a 3.16-fold increased risk of chronic HBV infection. This study suggested that IL-12A rs568408 and gene-gene interactions of IL-12A rs568408 and IL-12B rs3212227 contributed to the outcome of chronic HBV infection, meanwhile indicating their usefulness as a predictive and diagnostic biomarker of chronic HBV infection.
鉴于白细胞介素-12（IL-12）在乙型肝炎病毒（HBV）控制中的关键作用，我们研究了白细胞介素-12A rs568408 和白细胞介素-12B rs3212227 多态性与突尼斯人群慢性 HBV 感染风险之间的可能相关性。使用 PCR-RFLP 对 200 例慢性 HBV 感染患者和 200 例健康对照进行基因分型。与对照组相比，慢性 HBV 感染组中白细胞介素-12A rs568408 的 A 等位基因、AA 和 AG 基因型更为常见，分别与发生这种感染的 1.65 倍、2.58 倍和 3.13 倍的风险相关。基因-基因相互作用分析表明，携带白细胞介素-12A rs568408AA/AG 和白细胞介素-12B rs3212227AA 基因型的受试者发生慢性 HBV 感染的风险增加了 3.16 倍。这项研究表明，白细胞介素-12A rs568408 以及白细胞介素-12A rs568408 和白细胞介素-12B rs3212227 的基因-基因相互作用导致了慢性 HBV 感染的结果，同时表明它们可用作慢性 HBV 感染的预测和诊断生物标志物。
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