IL-12A基因中的rs568408变异与伊拉克患者感染COVID-19的风险相关。
The rs568408 variant in the IL-12A gene is associated with risk for COVID-19 in Iraqi patients.
作者信息
Al-Khikani Falah Hasan Obayes, Alkhafaji Zaytoon Abdulridha
机构信息
Department of Microbiology, College of Medicine, University of Babylon, Hilla, Iraq.
Department of Microbiology, Al-Shomali General Hospital, Babylon Directorate Health, Babylon, Iraq.
出版信息
Tzu Chi Med J. 2022 Dec 7;35(2):152-157. doi: 10.4103/tcmj.tcmj_223_22. eCollection 2023 Apr-Jun.
OBJECTIVES
The objective of the current study was to check the link between potential polymorphism in IL12A rs568408 and the possible risk of COVID-19 in the Iraqi population.
MATERIALS AND METHODS
Allele specific-polymerase chain reaction (PCR) technique was carried out for genotyping and detection of IL12A rs568408 gene polymorphism in a case-control study of 125 severe COVID-19 cases and 60 controls. Patients were admitted to either Marjan medical city or Al-Sadeq hospital's COVID-19 wards between January and June 2022 in Iraq. The diagnosis of COVID-19 in each patient was confirmed by severe acute respiratory coronavirus 2-positive reverse transcription-PCR.
RESULTS
The distribution of both genotyping and allele frequencies of IL-12A rs568408 revealed significant differences between patients and control groups ( = 0.006 and = 0.001, respectively). The IL12A rs568408 AA and AG variant genotypes were associated with a significantly increased risk of COVID-19 (odds ratio [OR] = 5.19, 95% confidence interval [CI]: 1.13-23.82; = 0.034) and (OR = 2.39, 95% CI = 1.16-4.94, = 0.018), respectively, compared with the wild-type GG homozygote.
CONCLUSION
These findings indicate that IL12A rs568408 GA/AA variant may contribute to the risk of COVID-19. This study is the first report about the association of IL12A rs568408 with COVID-19.
目的
本研究的目的是检测白细胞介素12A（IL12A）基因rs568408位点的潜在多态性与伊拉克人群感染新型冠状病毒肺炎（COVID-19）的潜在风险之间的联系。
材料与方法
在一项病例对照研究中，对125例重症COVID-19患者和60例对照者采用等位基因特异性聚合酶链反应（PCR）技术进行基因分型和检测IL12A rs568408基因多态性。2022年1月至6月期间，伊拉克的患者被收治于马尔詹医疗城或萨迪克医院的COVID-19病房。通过严重急性呼吸综合征冠状病毒2阳性逆转录PCR确诊每位患者的COVID-19。
结果
IL-12A rs568408的基因分型和等位基因频率分布在患者组和对照组之间存在显著差异（分别为P = 0.006和P = 0.001）。与野生型GG纯合子相比，IL12A rs568408的AA和AG变异基因型与COVID-19风险显著增加相关（优势比[OR] = 5.19，95%置信区间[CI]：1.13 - 23.82；P = 0.034）和（OR = 2.39，95% CI = 1.16 - 4.94，P = 0.018）。
结论
这些发现表明IL12A rs568408的GA/AA变异可能与COVID-19风险有关。本研究是关于IL12A rs568408与COVID-19关联的首次报道。
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