Ye Zi, Sun Tongrui, Hao Huoye, He Yanling, Liu Xueyan, Guo Minrui, Chen Guogang
School of Food Science and Technology, College of Food, Shihezi University, Shihezi, 832000, China.
AMB Express. 2021 Nov 14;11(1):149. doi: 10.1186/s13568-021-01313-6.
Rhodosporidium toruloides is a useful oleaginous yeast, but lipids production is affected by various factors including nutrients in the culture medium. Herein, the R-ZL2 high-yield mutant strain was used to investigate the effects of different carbon sources (sucrose, glucose, xylose), nitrogen sources (ammonium sulphate, ammonium nitrate), and C/N ratio on lipids production capacity, get the following conclusion (1) Compared with glucose and xylose, sucrose was a superior carbon source for lipids production; (2) When using ammonium sulphate as the nitrogen source, a C/N ratio of 200:1 achieved the highest biomass, lipids production and lipids content (10.7 g/L, 6.32 g/L and 59%, respectively), and lipids produced under different C/N conditions have potential for biodiesel production (except for C/N = 40 and C/N = 80); (3) When using ammonium nitrate as the nitrogen source, a C/N ratio of 200:1 achieved the highest biomass, lipids production and lipids content (12.1 g/L, 8.25 g/L and 65%, respectively), and lipids produced under different C/N ratio conditions have potential for biodiesel production. Thus, a combination of sucrose and ammonium nitrate was optimal for the lipid accumulation in R-ZL2. The findings will lay a foundation for further improving lipids yields.
红冬孢酵母是一种有用的产油酵母，但脂质产量受多种因素影响，包括培养基中的营养成分。在此，使用R-ZL2高产突变菌株研究不同碳源（蔗糖、葡萄糖、木糖）、氮源（硫酸铵、硝酸铵）和碳氮比对脂质生产能力的影响，得出以下结论：（1）与葡萄糖和木糖相比，蔗糖是脂质生产的优质碳源；（2）以硫酸铵作为氮源时，碳氮比为200:1时生物量、脂质产量和脂质含量最高（分别为10.7 g/L、6.32 g/L和59%），不同碳氮比条件下产生的脂质具有生物柴油生产潜力（碳氮比为40和80时除外）；（3）以硝酸铵作为氮源时，碳氮比为200:1时生物量、脂质产量和脂质含量最高（分别为12.1 g/L、8.25 g/L和65%），不同碳氮比条件下产生的脂质具有生物柴油生产潜力。因此，蔗糖和硝酸铵的组合对R-ZL2中的脂质积累最为有利。这些发现将为进一步提高脂质产量奠定基础。
