The United Graduate School of Veterinary Science, Yamaguchi University, Yoshida 1677-1, Yamaguchi-shi, Yamaguchi 753-8515, Japan.
J Vet Med Sci. 2020 Aug 19;82(8):1113-1117. doi: 10.1292/jvms.20-0159. Epub 2020 Jun 17.
Cow fertility decreases with age, but the hypothalamic pathomechanisms are not understood. Anti-Müllerian hormone (AMH) stimulates gonadotropin-releasing hormone (GnRH) neurons via AMH receptor type 2 (AMHR2), and most GnRH neurons in the preoptic area (POA), arcuate nucleus (ARC), and median eminence (ME) express AMH and AMHR2. Therefore, we hypothesized that both protein amounts would differ in the anterior hypothalamus (containing the POA) and posterior hypothalamus (containing the ARC and ME) between young post-pubertal heifers and old cows. Western blot analysis showed lower (P<0.05) expressions of AMH and AMHR2 in the posterior hypothalamus, but not in the anterior hypothalamus, of old Japanese Black cows compared to young heifers. Therefore, AMH and AMHR2 were decreased in the posterior hypothalami of old cows.
奶牛的生育能力会随着年龄的增长而下降，但下丘脑的病理机制尚不清楚。抗缪勒管激素（AMH）通过 AMH 受体类型 2（AMHR2）刺激促性腺激素释放激素（GnRH）神经元，而大多数位于视前区（POA）、弓状核（ARC）和正中隆起（ME）的 GnRH 神经元表达 AMH 和 AMHR2。因此，我们假设在年轻的产后小母牛和老年奶牛之间，前下丘脑（包含 POA）和后下丘脑（包含 ARC 和 ME）中这两种蛋白质的含量会有所不同。Western blot 分析显示，与年轻小母牛相比，老年日本黑牛的后下丘脑（但前下丘脑没有）中 AMH 和 AMHR2 的表达水平较低（P<0.05）。因此，老年奶牛的后下丘脑 AMH 和 AMHR2 减少。
