Kereilwe Onalenna, Kadokawa Hiroya
Faculty of Veterinary Medicine, Yamaguchi University, Yamaguchi-shi, Yamaguchi-ken 1677-1, Japan.
Reprod Fertil Dev. 2019 Apr;31(4):810-819. doi: 10.1071/RD18341.
Anti-Müllerian hormone (AMH) is secreted from ovaries and stimulates gonadotrophin secretion from bovine gonadotroph cells. Other important hormones for endocrinological gonadotroph regulation (e.g. gonadotrophin-releasing hormone, inhibin and activin) have paracrine and autocrine roles. Therefore, in this study, AMH expression in bovine gonadotroph cells and the relationships between AMH expression in the bovine anterior pituitary (AP) and oestrous stage, age and breed were evaluated. AMH mRNA expression was detected in APs of postpubertal heifers (26 months old) by reverse transcription-polymerase chain reaction. Based on western blotting using an antibody to mature C-terminal AMH, AMH protein expression was detected in APs. Immunofluorescence microscopy utilising the same antibody indicated that AMH is expressed in gonadotrophs. The expression of AMH mRNA and protein in APs did not differ between oestrous phases (P>0.1). We compared expression levels between old Holsteins (79.2±10.3 months old) and young (25.9±0.6 months old) and old Japanese Black females (89.7±20.3 months old). The APs of old Holsteins exhibited lower AMH mRNA levels (P<0.05) but higher AMH protein levels than those of young Japanese Black females (P<0.05). In conclusion, bovine gonadotrophs express AMH and this AMH expression may be breed-dependent.
抗苗勒管激素（AMH）由卵巢分泌，并刺激牛促性腺激素细胞分泌促性腺激素。其他对促性腺激素细胞进行内分泌调节的重要激素（如促性腺激素释放激素、抑制素和激活素）具有旁分泌和自分泌作用。因此，在本研究中，评估了牛促性腺激素细胞中AMH的表达，以及牛垂体前叶（AP）中AMH表达与发情阶段、年龄和品种之间的关系。通过逆转录-聚合酶链反应在青春期后小母牛（26月龄）的垂体前叶中检测到AMH mRNA表达。基于使用针对成熟C末端AMH的抗体进行的蛋白质印迹法，在垂体前叶中检测到了AMH蛋白表达。利用相同抗体的免疫荧光显微镜检查表明，AMH在促性腺激素细胞中表达。发情阶段之间，垂体前叶中AMH mRNA和蛋白的表达没有差异（P>0.1）。我们比较了老年荷斯坦奶牛（79.2±10.3月龄）与年轻（25.9±0.6月龄）和老年日本黑牛（89.7±20.3月龄）之间的表达水平。老年荷斯坦奶牛的垂体前叶AMH mRNA水平较低（P<0.05），但AMH蛋白水平高于年轻日本黑牛（P<0.05）。总之，牛促性腺激素细胞表达AMH，且这种AMH表达可能与品种有关。
