Department of Gynecology, Dongchangfu District, Liaocheng Dongchangfu People's Hospital, No. 128 Songgui Road, Liaocheng, 252000, Shandong, China.
Delivery Room, Dongchangfu District, Liaocheng Dongchangfu People's Hospital, No. 128 Songgui Road, Liaocheng, 252000, Shandong, China.
Mol Cell Biochem. 2020 Aug;471(1-2):177-188. doi: 10.1007/s11010-020-03778-8. Epub 2020 Jun 17.
Long non-coding RNA (lncRNA) Ewing sarcoma associated transcript 1 (EWSAT1) is an oncogene in a variety of tumors. Here, we planned to demonstrate EWSAT1 function in cervical cancer and further illustrate its underlying mechanism. EWSAT1 expression in cervical cancer was evaluated through qRT-PCR. Colony forming capacity was measured by colony formation assay and cell proliferation ability was measured by CCK-8 kit. Wound healing experiment was applied to test the cell migration and transwell assay was applied to test the invasion ability. Luciferase assay was employed to demonstrate EWSAT1 and miR-330-5p interaction. In cervical cancer, the expression of EWSAT1 was enhanced and contributed to the poor prognosis. Downregulated EWSAT1 expression inhibited Hela cell migration, proliferation, and invasion. EWSAT1 targeted to miR-330-5p and upregulated cytoplasmic polyadenylation element-binding protein 4 (CPEB4) expression by sponging miR-330-5p. Our study revealed that EWSAT1 enhances CPEB4 expression through sponging miR-330-5p, thereby promoting cervical cancer development, which might provide potential therapeutic targets for clinically cervical cancer patients.
长链非编码 RNA（lncRNA）尤文肉瘤相关转录物 1（EWSAT1）是多种肿瘤中的癌基因。在这里，我们计划证明 EWSAT1 在宫颈癌中的功能，并进一步阐明其潜在机制。通过 qRT-PCR 评估宫颈癌中 EWSAT1 的表达。通过集落形成测定法测量集落形成能力，通过 CCK-8 试剂盒测量细胞增殖能力。应用划痕愈合实验检测细胞迁移能力，应用 Transwell assay 检测侵袭能力。荧光素酶测定法用于证明 EWSAT1 和 miR-330-5p 的相互作用。在宫颈癌中，EWSAT1 的表达增强并导致预后不良。下调 EWSAT1 表达抑制 Hela 细胞迁移、增殖和侵袭。EWSAT1 通过海绵 miR-330-5p 靶向 microRNA-330-5p，并上调细胞质多聚腺苷酸化元件结合蛋白 4（CPEB4）的表达。我们的研究表明，EWSAT1 通过海绵 miR-330-5p 增强 CPEB4 的表达，从而促进宫颈癌的发展，这可能为临床上的宫颈癌患者提供潜在的治疗靶点。
