LncRNA EWSAT1 Promotes Colorectal Cancer Progression Through Sponging miR-326 to Modulate FBXL20 Expression.

BACKGROUND

Ewing sarcoma-associated transcript 1 () has been reported to be a pivotal modulator in a series of cancers. However, the function of in colorectal cancer (CRC) has not been elaborated. This study aimed to explore the role of in CRC progression and the underlying mechanisms.

METHODS

The expression patterns of , and were examined by qCRCR. Si-EWSAT1 was transfected to study the effects of on cell proliferation and metastasis. Rescue experiments were performed to investigate the underlying mechanisms in vitro. Xenograft models were used to evaluate the role of in vivo.

RESULTS

We found that was highly expressed in CRC tissues and cell lines and associated with poor overall survival. In vitro, knockdown of suppressed the cell proliferation, migration and invasion. Moreover, miR-326 was found to be a target of , and inhibitor could partially reverse the effects on CRC cell progression induced by si-EWSAT1. Subsequently, we validated as a vital downstream target for , and positively regulated via in vitro. In addition, these findings were confirmed by in vivo experiments.

CONCLUSION

Taken together, the data showed that promoted CRC progression via targeting pathway, which might provide a novel therapeutic target for CRC treatment.