Key Laboratory of Wetland Ecology and Environment, Northeast Institute of Geography and Agroecology, Chinese Academy of Sciences, Changchun, 130102, P R China.
University of Chinese Academy of Sciences, Beijing, 100049, P R China.
Sci Rep. 2020 Jun 19;10(1):10002. doi: 10.1038/s41598-020-66989-5.
A salt-tolerant denitrifying bacterium strain F2 was isolated from seawall muddy water in Dalian City, Liaoning Province, China. Strain F2 was identified by morphological observations, physiological and biochemical characteristics and 16 S rDNA identification. The salt tolerance of strain F2 was verified and the factors affecting the removal ability of strain F2 to nitrous nitrogen (NON) and nitrate nitrogen (NON) in saline conditions were investigated. Strain F2 was identified as Alishewanella sp., named Alishewanella sp. F2. Strain F2 can tolerate NaCl concentrations up to 70 g/L, and its most efficient denitrification capacity was observed at NaCl concentrations of 0-30 g/L. In the medium with NaCl concentrations of 0-30 g/L, strain F2 exhibited high removal efficiencies of NON and NON, with the removal percentages for both NON and NON of approximately 99%. In saline conditions with 30 g/L NaCl, the optimum culture pH, NaNO initial concentrations and inoculation sizes of strain F2 were 8-10, 0.4-0.8 g/L and 5-7%, respectively. Strain F2 was highly effective in removing NON and NON in saline conditions, and it has a good application potential in saline wastewater treatment.
一株耐盐反硝化细菌 F2 株从中国辽宁省大连市海堤泥水中分离得到。通过形态观察、生理生化特性和 16S rDNA 鉴定对菌株 F2 进行了鉴定。验证了菌株 F2 的耐盐性，并研究了盐度条件下影响菌株 F2 去除亚硝态氮（NON）和硝态氮（NON）能力的因素。菌株 F2 被鉴定为 Alishewanella sp.，命名为 Alishewanella sp. F2。菌株 F2 可耐受高达 70 g/L 的 NaCl，在 0-30 g/L 的 NaCl 浓度下其最有效的反硝化能力最高。在 0-30 g/L NaCl 的培养基中，菌株 F2 对 NON 和 NON 的去除率均较高，两者的去除率均接近 99%。在 30 g/L NaCl 的盐度条件下，菌株 F2 的最佳培养 pH 值、NaNO 初始浓度和接种量分别为 8-10、0.4-0.8 g/L 和 5-7%。菌株 F2 在盐度条件下对 NON 和 NON 的去除效果显著，在处理含盐废水方面具有良好的应用潜力。
