Laboratory of Microbial Cell Surface Biochemistry, G.K. Skryabin Institute of Biochemistry and Physiology of Microorganisms, Russian Academy of Sciences, PSCBR RAS, 5 Prosp. Nauki, Pushchino, Moscow Region, 142290, Russia.
Department of Proteomics, V.N. Orekhovich Research Institute of Biomedical Chemistry, Russian Academy of Medical Sciences, 10 Pogodinskaja Str., Moscow, 119832, Russia.
Sci Rep. 2020 Jun 19;10(1):9944. doi: 10.1038/s41598-020-67122-2.
Recent recurrent outbreaks of bacterial resistance to antibiotics have shown the critical need to identify new lytic agents to combat them. The species Lysobacter capsici VKM B-2533 possesses a potent antimicrobial action against a number of bacteria, fungi and yeasts. Its activity can be due to the impact of bacteriolytic enzymes, antibiotics and peptides. This work isolated four homogeneous bacteriolytic enzymes and a mixture of two proteins, which also had a bacteriolytic activity. The isolates included proteins identical to L. enzymogenes α- and β-lytic proteases and lysine-specific protease. The proteases of 26 kDa and 29 kDa and a protein identified as N-acetylglycosaminidase had not been isolated in Lysobacter earlier. The isolated β-lytic protease digested live methicillin-resistant staphylococcal cells with high efficiency (minimal inhibitory concentration, 2.85 μg/mL). This property makes the enzyme deserving special attention. A recombinant β-lytic protease was produced. The antimicrobial potential of the bacterium was contributed to by outer membrane vesicles (OMVs). L. capsici cells were found to form a group of OMVs responsible for antifungal activity. The data are indicative of a significant antimicrobial potential of this bacterium that requires thorough research.
最近，细菌对抗生素的耐药性反复爆发，这表明迫切需要确定新的裂解剂来对抗它们。辣椒地杆菌（Lysobacter capsici）VKM B-2533 株具有针对多种细菌、真菌和酵母的强大抗菌作用。其活性可能归因于溶菌酶、抗生素和肽的影响。本研究分离出四种均一的溶菌酶和两种具有溶菌活性的蛋白质混合物。分离物包括与 L. enzymogenes α-和 β-溶菌蛋白酶以及赖氨酸特异性蛋白酶相同的蛋白质。以前在 Lysobacter 中尚未分离到 26 kDa 和 29 kDa 的蛋白酶以及被鉴定为 N-乙酰氨基葡萄糖苷酶的蛋白质。分离出的 β-溶菌蛋白酶能高效（最小抑菌浓度 2.85μg/mL）分解活的耐甲氧西林金黄色葡萄球菌细胞。这种特性使其成为值得特别关注的酶。还生产了重组 β-溶菌蛋白酶。细菌的抗菌潜力归因于外膜囊泡（OMVs）。发现辣椒地杆菌细胞形成了一组负责抗真菌活性的 OMVs。这些数据表明该细菌具有重要的抗菌潜力，需要进行深入研究。
